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微小RNA-147、Ki-67及癌胚抗原表达水平与浸润性肺腺癌淋巴结转移的关系

Correlations of expression levels of microRNA-147, Ki-67 and carcinoembryonic antigen with lymph node metastasis in invasive pulmonary adenocarcinoma

    摘要
  • 摘要:
    目的 探讨微小RNA-147(miR-147)、Ki-67以及癌胚抗原(CEA)表达水平与浸润性肺腺癌淋巴结转移的关系。
    方法 选取浸润性肺腺癌患者160例为研究对象, 根据患者淋巴结转移情况，分为有淋巴结转移组(n=74)和无淋巴结转移组(n=86)。采用免疫组化法检测患者癌组织中miR-147、Ki-67的表达水平。采用化学发光法检测患者血清CEA水平。分析miR-147和Ki-67以及血清CEA表达水平与患者临床病理特征的关系。采用受试者工作特征(ROC)曲线分析miR-147、Ki-67、CEA预测淋巴结转移的价值。
    结果 有淋巴结转移组的miR-147表达水平低于无淋巴结转移组, Ki-67、血清CEA表达水平高于无淋巴结转移组，差异有统计学意义(P < 0.05)。肿瘤直径≥3 cm、TNM分期为Ⅲ~Ⅳ期患者的癌组织miR-147表达水平低于肿瘤直径 < 3 cm、TNM分期为Ⅰ~Ⅱ期患者，差异有统计学意义(P < 0.05)。肿瘤直径≥3 cm患者的癌组织Ki-67表达水平高于肿瘤直径 < 3 cm患者，差异有统计学意义(P < 0.05)。TNM分期为Ⅲ~Ⅳ期患者的血清CEA水平高于TNM分期为Ⅰ~Ⅱ期患者，差异有统计学意义(P < 0.05)。miR-147表达水平与Ki-67、血清CEA、淋巴结转移呈负相关(P < 0.05); Ki-67、血清CEA与淋巴结转移呈正相关(P < 0.05); Ki-67与血清CEA呈正相关(P < 0.05)。miR-147、Ki-67、CEA预测淋巴结转移的ROC曲线的曲线下面积分别为0.785、0.835和0.798(P < 0.05)。
    结论 癌组织miR-147、Ki-67表达水平以及血清CEA水平与浸润性肺腺癌淋巴结转移存在相关性。

     

    Abstract:
    Objective To explore correlations of expression levels of microRNA-147 (miR-147), Ki-67 and carcinoembryonic antigen (CEA) with lymph node metastasis of invasive lung adenocarcinoma.
    Methods A total of 160 patients with invasive pulmonary adenocarcinoma were selected as study subjects. Based on presence of lymph node metastasis, patients were divided into lymph node metastasis group (n=74) and non-lymph node metastasis group (n=86). Immunohistochemistry was employed to detect the expression levels of miR-147 and Ki-67 in cancer tissues. The serum CEA level was measured using the chemiluminescence method. The correlations of the expression levels of miR-147, Ki-67 and serum CEA with the clinicopathological characteristics of patients were analyzed. The value of miR-147, Ki-67 and CEA in predicting lymph node metastasis was evaluated using the receiver operating characteristic (ROC) curve.
    Results The expression level of miR-147 in the lymph node metastasis group was significantly lower than that in the non-lymph node metastasis group, while the expression levels of Ki-67 and serum CEA were significantly higher in the lymph node metastasis group than in the non-lymph node metastasis group (P < 0.05). Patients with a tumor diameter ≥3 cm or TNM stage Ⅲ to Ⅳ had significantly lower miR-147 expression levels in cancer tissues compared to those with a tumor diameter < 3 cm or TNM stage Ⅰ to Ⅱ (P < 0.05). The Ki-67 expression level in cancer tissues was significantly higher in patients with a tumor diameter≥3 cm than in those with a tumor diameter < 3 cm (P < 0.05). The serum CEA level was significantly higher in patients with TNM stage Ⅲ to Ⅳ than in those with TNM stage Ⅰ to Ⅱ (P < 0.05). The expression level of miR-147 was negatively correlated with Ki-67, serum CEA and lymph node metastasis (P < 0.05); Ki-67 and serum CEA were positively correlated with lymph node metastasis (P < 0.05); Ki-67 was positively correlated with serum CEA (P < 0.05). The areas under the ROC curves for miR-147, Ki-67 and CEA in predicting lymph node metastasis were 0.785, 0.835 and 0.798, respectively (P < 0.05).
    Conclusion The expression levels of miR-147 and Ki-67 in cancer tissues and the serum CEA level are correlated with lymph node metastasis in invasive pulmonary adenocarcinoma.

     

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