Difference analysis of Liuwei Dihuang Granule on the gene expression profile of patients with colorectal cancer due to yin deficiency of liver and kidney

Objective The genomics mechanism of Liuwei Dihuang Granule efficacy was ex-plored by selecting the different gene expression profiles before and after the application of Liuwei Di-huang Granule for patients with advanced colorectal cancer (CRC)due to yin deficiency of liver and kidney.Methods Liuwei Dihuang Granule was given to patients with advanced CRC due to yin defi-ciency of liver and kidney,21 days as a course.During treatment,no western medicines or other Chi-nese medicines were administrated.21 days later,peripheral blood was collected again to detect the gene chips.Then the chips after intervention were compared with themselves before intervention.Re-sults Compared with intervention before,the chips after intervention had 129 differential gene frag-ments,in which 128 were up-regulated and 1 was down-regulated.Gene function enrichment anal-ysis showed that a total of 254 gene ontologies (GO)had significant differences before and after inter-ventions (P<0.05 orP<0.01).In the biological process,coagulation-related genes accounted for 41.5%.In cellular components,45.5%differential cells was associated with plasma membrane while in molecular function,64.9% was correlated with combination.Conclusion Patients with advanced CRC due to yin deficiency of liver and kidney have differential gene expressions before and after the in-terventions of Liuwei Dihuang Granule,and GO analysis indicates that Liuwei Dihuang Granule can strengthen the coagulation function and combination with calcium of CRC patients.