Establishment and Characterization of 5-Fu-resistant Variant of Human Pancreatic Cancer Cell Line
-
摘要: 目的 建立稳定传代的抵抗5-Fu的人胰腺癌细胞株MiaPaca2-5-Fu, 并对细胞生物学特性进行研究.方法 逐步增加培养基中5-Fu的浓度,建立对5-Fu耐药的人胰腺癌细胞株MiaPaca2-5-Fu;采用WST法计算出MiaPaca2和MiaPaca 2-5-Fu的半数抑制浓度(IC50)和耐药指数(RI);检测MiaPaca2和MiaPaca2-5-Fu的生长曲线,计算2个细胞系的倍增时间并进行比较,用流式细胞仪技术检测其细胞周期.结果 5-Fu对MiaPaca2和MiaPaca2-5-Fu的IC50分别为(4.29±0.15) μg/mL、(41.55±2.79) μg/mL, RI为9.68(P=0.0019).根据生长曲线计算出MiaPaca2和MiaPaca2-5-Fu的倍增时间分别为(39.52±0.32) h、(43.27±0.33) h (P=0.0069), 2细胞株的G0/G1期、S期、G2/M期均存在显著性差异(P＜0.01).结论 成功建立了对5-Fu耐药的人胰腺癌细胞系MiaPaca2-5-Fu, 耐药性能明显、稳定,适合于胰腺癌中5-Fu耐药机制的研究.
-
-
Philadelphia P A, Lippincott Williams, Wilkins, DeVita VT, H S Rosenberg SA. Oncology [M]. 2001.1126. Berlin J D, Rothenberg M L. Chemotherapeutic advances in pancreatic cancer [J]. Current Oncology Reports, 2003(3):219.doi: 10.1007/s11912-003-0113-8. Stavrovskaya A A. Cellular mechanisms of multidrug resistance of tumor cell [J]. 2000.95. Flens M J, Scheffer G L, Van Der Valk P. Identification of novel drug resistance-associated by a panel of rat monoclonal antibodies [J]. International Journal of Cancer, 1997(2):249.doi: 10.1002/(SICI)1097-0215(19971009)73:2<249::AID-IJC15>3.0.CO;2-A. Snow K, Judd W. Characteristics of adriamycin-and amsacrine-resistant human leukaemic T cell line [J]. British Journal of Cancer, 1991(1):17. Ishii K, Iwahana M, Mitsui I. Growth inhibitory effect of a new camptothecin analog, DX-8951f, on various drug-resistant sublines including BCRP-mediated camptothecin derivative-resistant variants derived from the human lung cancer line PC-6 [J]. Anti-Cancer Drugs, 2000(5):356.doi: 10.1097/00001813-200006000-00005. Bergman A M, Giaccone G, Van Moorsel G J. Cross-resistance in the 2', 2'-difluro-deoxycydine(gemicitabine):resistant human ovarian cancer cell line AG6000 to standard and investigational drug [J]. European Cancer, 2000.1974. Umezu Goto M, Kishi Y, Taiva A. Autotaxin has lysophosphlipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production [J]. Journal of Cell Biology, 2002(2):227. Hsieh J L, Wu C L, Lee C H. Hepatitis B virus X protein sensitizes hepatocellular carcinoma cells to cytolysis induced by E1B-deleted adenovirus through the disruption of P53 FUnction [J]. Clinical Cancer Research, 2003(1):338. -
期刊类型引用(7)
1. 郭振，胡晓萌，曹海玮，时玉春，黄旭，张冲. 超声联合螺旋CT对小儿髋关节发育异常的诊断价值. 临床医学. 2024(01): 90-92 . 
百度学术
2. 李凯，夏伊明，单新平，吴鹏，阿尔帕提·努力买买提，石岩. 伊犁地区婴幼儿发育性髋关节发育不良的危险因素分析. 公共卫生与预防医学. 2024(03): 157-160 . 百度学术
3. 杨茜，石影，张永花，郑华，张婧洁，刘艳敏. 婴儿发育性髋关节发育不良超声筛查情况及影响因素分析. 兰州大学学报(医学版). 2024(07): 37-42 . 百度学术
4. 蔡如意，陈敏. 早产儿髋关节发育不良的临床特征、超声表现及相关因素分析. 转化医学杂志. 2024(05): 696-701 . 百度学术
5. 何花，王鑫，林海庆，桂冬冬，孙泉，许子旻. 高频超声在婴幼儿髋关节发育异常早期诊断中的价值分析. 现代诊断与治疗. 2023(08): 1231-1232+1238 . 百度学术
6. 弓建志. 临夏0～2岁婴幼儿发育性髋关节发育不良筛查暨影响因素分析. 智慧健康. 2023(26): 52-55 . 百度学术
7. 陈浩，孟晓涛，李晓婷，李敏，李洪涛，任超，刘春燕. 包头地区婴幼儿发育性髋关节发育不良的超声动静态法筛查结果分析. 航空航天医学杂志. 2022(11): 1308-1311 . 百度学术
其他类型引用(1)
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
- 被引次数: 8
下载:
苏公网安备 32100302010246号