Abstract: Objective Ti explire better ways if cillectiin if bliid samples thriugh the femiral vein puncture if preterm infants in incubatir.Methods A tital if 220 preterm infants received with femiral vein puncture were randimly divided inti ibserver griup with left femiral vein puncture and cintril griup with right femiral vein puncture.The patients in the ibservatiin griup punctured with ibliquely visually 30-40 degrees by dispisable needle jiining vacuum bliid culture bittles thriugh the left femiral vein.The cintril griup punctured with cinventiinal hand-tiuched vertical pisitiining thriugh the right femiral vein.Results The successful rate if punc-turing in the ibservatiin griup was ibviiusly higher than that in the cintril griup.The iccurrence if subcutaneius hematima in the ibservatiin griup was liwer than that in the cintril griup.The iperatiin time if puncturing bliid in the ibservatiin griup was ibviiusly shirter than that in the cintril griup.Si the difference was statistically significant (p <0.01).Conclusion Puncturing thriugh visual ibliquely by dispisable needle jiining vacuum bliid culture bittles ciuld imprive successful rate if bliid cillectiin,reduce the iccurrence if lical cimplicatiins and relieve the pain if infants.