Clinical significance and expression ofβdefensin-2 of colonic mucosa in patients with ulcerative colitis
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摘要: 目的：探讨溃疡性结肠炎患者结肠黏膜β防御素-2（HBD-2）的表达及临床意义。方法2009年5月-2012年12月本院收治的溃疡性结肠炎患者30例为溃疡性结肠炎组，肠易激综合征患者30例为肠易激综合征组，另选择健康体检者30例为对照组。3组患者入院均行电子结肠镜检查，收集到的活检组织一部分立即提取 RNA 用于 PT-PCR 检测，另一部分进行HBD-2免疫组织化学染色（SP 法）。比较各组结肠黏膜 HBD-2 mRNA 表达水平、结肠黏膜中 HBD-2表达阳性率、结肠黏膜中HBD-2表达强度。结果溃疡性结肠炎组、肠易激综合征组患者结肠黏膜 HBD-2 mRNA 水平均明显高于对照组。溃疡性结肠炎组患者结肠黏膜 HBD-2 mRNA 水平明显高于肠易激综合征组。溃疡性结肠炎组结肠黏膜 HBD-2阳性表达率明显高于肠易激综合征组、对照组。溃疡性结肠炎组结肠黏膜中 HBD-2表达强度（2＋、3＋）明显强于肠易激综合征组、对照组。结论 HBD-2在溃疡性结肠炎的发生发展中发挥重要作用。Abstract: Objective To explore the clinical significance and expression ofβdefensin-2 of colonic mucosa in patients with ulcerative colitis (UC).Methods 30 patients with UC enrolled from May 2009 to December 2012 in our hospital were selected as UC group,30 patients with irri-table bowel syndrome (IBS)were in IBS group and 30 healthy people were in control group.Elec-tronic colonoscopy was applied in all groups.RNA was extracted immediately from part of the col-lected biopsy tissues for PT-PCR detection,and the rest biopsy tissues were used for HBD-2 im-munohistochemical staining by SP method.HBD-2 mRNA expression level,positive rate and inten-sity of HBD-2 of colonic mucosa in patients of three groups were compared.Results HBD-2 mRNA levels in UC and IBS groups were evidently higher than that in the control group,while patient in UC group had the highest level.And the levels were obviously higher in HBD-2 positive expression rate and HBD-2 expression intensity (2+,3+)in colonic mucosa than that in other two groups. Conclusion HBD-2 plays an important role in the development and progression of UC.
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Keywords:
- ulcerative colitis /
- irritable bowel syndrome /
- βdefensin-2
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期刊类型引用(2)
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