Abstract: Objective To study the distribution and the subtypes of human papillomavirus (HPV)infection of female patients with cervical cancer and /or other genital diseases in Gansu province,attempting to provide virological data for the prevention of HPV infection and treatment of cervical cancer.Methods A total of 164 specimens with cervical cancer and 100patients with oth-er genital diseases were detected by flow-through hybridization and gene chip.Results 14 HPV subtypes were detected in the 164 patients with cervical cancer (positive HPV DNA rate:85.4%), in which the 10 HPV subtypes with high risk were HPV-16 (68.3%,112 /164),-52 (6.1%,10 /164),-58,-18,-33 (3.7% each 6 /164),-59 (2.4%,4 /164)and-31,-45 (1.2% each,2 /164) while 4 HPVsubtypesoflowriskwereHPV-6 (1 .2 % ,2 /1 6 4 ),-1 1 (4 .9 % ,8 /1 6 4 ),-4 2 (1.2%,2 /164)and-CP8304 (2.4%,4 /164).9 HPV subtypes were detected in 100 patients with other genital diseases (positive HPV DNA rate:28.0%),in which 8 HPV subtypes with high risk were HPV-16 (12%,12 /100),-52 (6.0%,6 /100),-68 (4.0%,4 /100),-31,-18,-39,-58, and-59 (2.0%each,2 /100)while 1 HPV subtype with low risk was HPV-11 (8.0%,8 /100). Multiple infection rate of cervical cancer was 17.1%(28 /164)and multiple infection rate of genital tractwas6 .0 % (6 /1 0 0 ).Conclusion High risk HPV subtypes infection is the dominant factor for cervical cancer in Gansu province ,in which HPV-1 6 and HPV-5 2 are the most common subtypes.There are certain correlation between HPV infection and other genital diseases.The flow-through hybridization and gene chip technique can be applied to detect HPV of female pa-tients with genital infection to confirm subtype genotypes of HPV and to guide clinic treatment and follow-up visits.