Difference analysis of Liuwei Dihuang Granule on the gene expression profile of patients with colorectal cancer due to yin deficiency of liver and kidney
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摘要: 目的:筛选肝肾阴虚型晚期结直肠癌(CRC)患者使用六味地黄颗粒前后的基因表达谱差异,探索六味地黄颗粒药效的基因组学机制。方法选取晚期肝肾阴虚型CRC患者以六味地黄颗粒进行干预,21 d为1个疗程。干预期间患者不接受其他西医及中医药治疗。21 d后再次提取外周血,进行基因芯片检测,并与干预前芯片结果进行自身对照分析。结果与干预前相比,干预后基因芯片检测发现129个差异基因条,其中128个上调,1个下调。基因功能(GO)富集分析结果显示,干预前后共发现254个基因GO存在显著差异(P<0.05或P<0.01)。在生物过程中,凝血功能相关的基因占41.5%;在细胞组成中,45.5%的差异基因与细胞质膜有关;在分子功能方面,64.9%的差异基因与结合有关。结论晚期CRC肝肾阴虚型患者应用六味地黄颗粒干预前后存在差异表达基因,功能分析显示六味地黄颗粒可增强患者凝血功能,增加钙离子结合。Abstract: Objective The genomics mechanism of Liuwei Dihuang Granule efficacy was ex-plored by selecting the different gene expression profiles before and after the application of Liuwei Di-huang Granule for patients with advanced colorectal cancer (CRC)due to yin deficiency of liver and kidney.Methods Liuwei Dihuang Granule was given to patients with advanced CRC due to yin defi-ciency of liver and kidney,21 days as a course.During treatment,no western medicines or other Chi-nese medicines were administrated.21 days later,peripheral blood was collected again to detect the gene chips.Then the chips after intervention were compared with themselves before intervention.Re-sults Compared with intervention before,the chips after intervention had 129 differential gene frag-ments,in which 128 were up-regulated and 1 was down-regulated.Gene function enrichment anal-ysis showed that a total of 254 gene ontologies (GO)had significant differences before and after inter-ventions (P<0.05 orP<0.01).In the biological process,coagulation-related genes accounted for 41.5%.In cellular components,45.5%differential cells was associated with plasma membrane while in molecular function,64.9% was correlated with combination.Conclusion Patients with advanced CRC due to yin deficiency of liver and kidney have differential gene expressions before and after the in-terventions of Liuwei Dihuang Granule,and GO analysis indicates that Liuwei Dihuang Granule can strengthen the coagulation function and combination with calcium of CRC patients.
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