核因子κB 65及黏附分子在妊娠高血压综合征患者胎盘组织中的表达及意义

牟华平; 袁志平; 陈佳莲; 邓黎

doi:10.7619/jcmp.201417019

核因子κB 65及黏附分子在妊娠高血压综合征患者胎盘组织中的表达及意义

1.
四川省宜宾市第二人民医院妇产科，四川宜宾，644000;
2.
四川省宜宾市第二人民医院肿瘤科，四川宜宾，644000

基金项目: 中国高校医学期刊临床专项资金

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中图分类号: R714.24
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- 发布日期: 2014-10-16

Expression and significance of nuclear factorκB 65 and adhesion molecules in the placenta tissue of patients with pregnancy-induced hypertension syndrome

摘要

摘要: 目的：观察核因子κB 65（NF-κB65）、细胞间黏附分子-1（ICAM-1）及血管细胞黏附分子-1（VCAM-1）在妊娠高血压综合征（PIH）患者胎盘组织中的表达及其临床意义。方法选取住院分娩的PIH患者58例设为PIH组，同期正常产妇40例设为对照组，免疫组化法检测2组胎盘组织绒毛毛细血管内皮细胞及滋养细胞内NF-κB65、ICAM-1、VCAM-1的表达。结果PIH组各指标阳性表达率和表达强度值与对照组差异显著（P＜0．05或P＜0．01），且阳性表达率及表达强度均随着病情加重呈现出逐步增加或降低的趋势；相关性分析表明，在毛细血管内皮细胞中，PIH患者的NF-κB65表达强度与ICAM-1、VCAM-1呈显著正相关（r＝0．951，0．894，P＜0．01）；在绒毛滋养细胞中，NF-κB65表达强度与ICAM-1表达强度呈显著正相关（r＝0．801，P＜0．01）。结论 PIH患者胎盘组织绒毛毛细血管内皮细胞及滋养细胞内NF-κB65、ICAM-1、VCAM-1的表达显著升高或降低，NF-κB65的激活可能调控ICAM-1、VCAM-1的表达并参与PIH的发生发展。
- 妊娠高血压综合征 /
- 核因子κB /
- 细胞间黏附分子 /
- 血管细胞黏附分子 /
- 绒毛毛细血管内皮细胞 /
- 滋养细胞
Abstract: Objective To observe the expression and clinical significance of nuclear factorκB 65 (NF-κB65 ),intercellular adhesion molecule-1 (ICAM-1 )and vascular cell adhesion molecule-1 (VCAM-1 )in the placenta tissue of patients with pregnancy-induced hypertension syndrome (PIH). Methods 58 PIH patients received institutional delivery were designed as PIH group and 40 normal materials of the same period were designed as control group.Expression of NF-κB65、ICAM-1 and VCAM-1 in endothelial cells and trophoblast cells from villous capillary of placenta tissue of both groups were determined by using immunohistochemistry.Results The positive expression rate and ex-pression intensity in PIH group were significantly better than control group (P<0.05 or P<0.01 ). While the positive expression rate and expression intensity gradually increased with aggravation of PIH. Correlation analysis showed the expression intensity of NF-κB65 was significantly and positively correlated with that of ICAM-1 and VCAM-1 in capillary endothelial cells of PIH patients (r =0 .9 5 1 , 0.894,P<0.01 ).The expression intensity of NF-κB65 was significantly and positively correlated with that of ICAM-1 in villous trophoblast cells (r=0.801,P<0.01).Conclusion The expression of NF-κB 6 5 ,ICAM-1 and VCAM-1 increases or decreases significantly in placental capillary endothelial cells and trophoblastic cells of PIH patients.Activation of NF-κB65 may regulate the expression of ICAM-1、VCAM-1 and involved in the development of PIH.
- pregnancy-induced hypertension syndrome /
- nuclear factor κB 65 /
- intercel-lular adhesion molecule /
- vascular cell adhesion molecule /
- placental villi capillary endothelial cells /
- trophoblast cells

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核因子κB 65及黏附分子在妊娠高血压综合征患者胎盘组织中的表达及意义

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Expression and significance of nuclear factorκB 65 and adhesion molecules in the placenta tissue of patients with pregnancy-induced hypertension syndrome

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