Objective  To observe changes of refractive status, axis oculi and sclera morphology in guinea pigs and to explore the effect of Wuzi Yanzong Recipe on changes of scleral morphology in guinea pig with form-deprivation myopia.

  Methods  A total of 100 healthy guinea pigs with 2 weeks of age were selected, and deprivation myopia models were prepared by eye mask covering for 42 days. Their binocular diopter and axis oculi were recorded. The successful models were randomly divided into control group and treatment group, and their binocular diopter and axis oculi were recorded again. The treatment group applied intragastric administration of Wuzi Yanzong Recipe at a dose of 16 g/kg, once daily for 30 consecutive days. No intervention was performed in the control group. The sclera ultrastructure of the treatment group and the control group were examined and compared by transmission electron microscopy.

  Results  The right eyes of guinea pigs were successfully induced for form-deprivation myopia model after 42 d covering. Compared with the self-controlled eyes, the eye axis and diopter were significantly increased (P < 0.05). The electron microscope results showed that posterior scleral morphology in the treatment group showed some pathological changes, but showed anobvious changes than those in the control group.

  Conclusion  Wuzi Yanzong Recipe can delay progression of myopia by postponing the changes of scleral tissue morphology.