Value of allogeneic adipose stem cells combined with demineralized bone matrix in rabbit model of critical bone defect of radius
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摘要:目的 探讨同种异体脂肪干细胞复合脱钙骨基质对桡骨临界骨缺损兔模型的价值。方法 纳入18只成年雄性大白兔，切取双上肢桡骨干中段部分骨质，制备双侧上肢桡骨临界骨缺损模型。将18只兔子随机分为对照组、实验组1和实验组2。对照组未予任何干预，实验组1在骨缺损部位植入脱钙骨基质，实验组2在骨缺损部位植入同种异体脂肪干细胞复合脱钙骨基质。第8、12周时行X线、病理切片和解剖学检查，明确骨缺损的修复效果。结果 骨缺损修复后, 8、12周时对桡骨行X线检查，对照组骨缺损修复差; 8周时实验组1部分修复, 12周时大部分修复，但仍有1只兔子存在小块骨缺损; 8周时实验组2大部分修复, 12周时全部修复，实验组2可见髓腔通畅。8、12周时，实验组1和实验组2的Lane-Sandhu组织学评分均显著高于对照组，实验组2的Lane-Sandhu组织学评分均显著高于实验组1(P < 0.05)。造模12周后，对照组骨缺损区多为纤维组织，实验组1有编织骨形成，但骨小梁紊乱; 实验组2有板成骨形成，骨小梁排列紧密有序。结论 同种异体脂肪干细胞复合脱钙骨基质对桡骨临界骨缺损兔模型有一定的价值。Abstract:Objective To investigate the value of allogeneic adipose stem cells combined with demineralized bone matrix in rabbit model of critical bone defect of radius.Methods A total of 18 adult male rabbits were collected and treated with dissection of partial bone in the middle segment of the radial diaphysis of the upper limb, and the rabbit model of critical bone defect of radius of the bilateral upper limb was prepared. The 18 rabbits were randomly divided into control group, experimental group 1 and experimental group 2. The control group was not given any intervention, and the experimental group 1 was implanted with the demineralized bone matrix in the bone defect site, while the experimental group 2 was implanted with the allograft adipose stem cells and the decalcified bone matrix. The X-ray, pathological section and anatomical examination were performed at 8 and 12 weeks to clarify the repair effect of bone defect.Results After repairing the bone defect, X-ray examination was performed on the radius at 8 and 12 weeks. The bone defect in the control group was poorly repaired. At 8 weeks, part of the bone defect was repaired in the experimental group 1, and most of the bone defect was repaired at 12 weeks, but there was still a small bone defect in a rabbit. At 8 weeks, most of the bone defect was repaired in the experimental group 2, and all of them were repaired at 12 weeks. At 8 and 12 weeks, the Lane-Sandhu histological scores of the experimental group 1 and group 2 were significantly higher than that of the control group, and the Lane-Sandhu histological score of the experimental group 2 was significantly higher than that of the experimental group 1 (P < 0.05). After 12 weeks, the bone defect area in the control group was mostly fibrous tissue, while in the experimental group 1, woven bone was formed, but bone trabecula was disordered. In the experimental group 2, plate osteogenesis was formed, and bone trabeculae were arranged tightly and orderly.Conclusion Allogeneic adipose stem cells combined with demineralized bone matrix have a certain therapeutic value in the rabbit model of critical bone defect of radius.
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Keywords:
- allogeneic adipose stem cells /
- radius /
- bone defect /
- demineralized bone matrix
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图 1 脂肪干细胞的培养及传代(放大倍数100倍)
A、B、C、D依次表示原代脂肪干细胞第1、3、5、7天; E、F、G、H依次表示第2代脂肪干细胞第3、5、7、10天; I、J依次代表第3代脂肪干细胞第7、10天
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图 6 桡骨缺损的解剖学观察
A、B、C分别为对照组0、8、12周桡骨缺损的形态; D、E、F分别为实验组1在0、8、12周桡骨缺损的形态; G、H、I分别为实验组2在0、8、12周桡骨缺损的形态
表 1 Lane-Sandhu组织学评分评价(x±s)
分 组别 n 8周时 12周时 对照组 6 1.86±0.65 2.32±0.81 实验组1 6 4.73±1.12* 8.47±1.62* 实验组2 6 6.12±1.53*# 11.47±1.47*# 与对照组比较, *P < 0.05;与实验组1比较, P < 0.05。 
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