Abstract: Objective To explore the value of echocardiography in assessment of left ventricular morphology and diastolic function in patients with left ventricular diastolic heart failure. Methods From February 2015 to February 2018, 45 patients with left ventricular diastolic heart failure in authors' hospital were selected as observation group, and 45 healthy subjects were selected as control group. Both groups were examined by echocardiography. The cardiac function indexes such as left ventricular ejection fraction, cardiac output and 6-minute walking test were compared between two groups. The left heart shape indexes such as left atrial diameter, left ventricular diameter, left ventricular posterior wall thickness, interventricular septal thickness and cardiac index were compared between two groups. The left ventricular diastolic function indexes such as the peak value of early diastolic flow velocity, end diastolic flow velocity, blood flow propagation velocity and reverse flow velocity were compared between two groups. Results The left ventricular ejection fraction, cardiac output and 6-min walking results in the observation group were significantly worse than those in the control group(P < 0.05). The diameter of left atrium, the thickness of left posterior wall and the thickness of interventricular septum in the observation group were significantly larger than those in the control group(P < 0.05). There were no significant differences between the two groups in left ventricular diameter and heart index(P > 0.05). The peak value of early diastolic velocity and reverse blood flow velocity in the observation group were significantly lower than those in the control group, and the peak value of end diastolic velocity and blood flow propagation velocity were significantly - higher than those in the control group(P < 0.05). Conclusion Echocardiography can accurately evaluate left ventricular shape and diastolic function in patients with left ventricular diastolic heart failure, and increase the diagnostic accuracy of disease.