Abstract: Objective To explore the effect of intraoperative pre-incubation nursing measures on change of body temperature in children with laparoscopic surgery. Methods A total of 200 children with laparoscopic surgery were randomly divided into observation group and control group, with 100 cases in each group. The control group was treated with conventional warming measures, while the observation was treated with pre-incubation nursing measures. The change of intraoperative body temperature, incidence of shiver and stress indexes were compared between the two groups. Results The body temperatures at the time points of 30 minutes after cutting skin and the end of operation in the observation group were significantly higher than those in the control group, while the incidence rate of shiver was significantly lower than the control group(P<0.05). The levels of adrenaline and noradrenaline in the observation group were significantly lower than those in the control group(P<0.05). The time of extubation and recovery of spontaneous respiration in the observation group were significantly shorter than those in the control group(P<0.05). Conclusion Intraoperative pre-incubation nursing measures can keep the body temperature constant during operation, reduce the incidence of stress reaction and shiver, and shorten the time of extubation.