Abstract: Objective To explore the value of urine test of eight indexes combined with quantitative urine culture in the diagnosis of urinary tract infection. Methods The clinical materials of 100 patients with suspected urinary tract infection were analyzed retrospectively. All the patients conducted with urine test of eight indexes as well as combination of urine test of eight indexes and quantitative urine culture, and the accuracy and diagnostic efficacy of the examination results were analyzed. Results The accuracy of urine test of eight indexes combined with quantitative urine culture was significantly higher than that of single urine test of eight indexes(P<0.05), and the sensitivity and specificity of urine test of eight indexes combined with quantitative urine culture were significantly higher than those of single urine test of eight indexes(P<0.05). Conclusion Urine test of eight indexes combined with quantitative urine culture has a high accuracy rate in the diagnosis of urinary tract infection.