Abstract: Objective To investigate the effect of different types of wash-free disinfectants and stability of ethanol content after opening so as to prepare for official implementation of Specification of Hand Hygiene for Healthcare Workers(WS/T 313-2019)in the near future. Methods Foam, gel and spray disinfectants were selected, and 0.5 to 5.0 mL of each disinfectant was used for hand disinfection field tests to evaluate the optimal amount for ≥1.00 mean kill log value. The initial ethanol content after opening and ethanol content at 2, 4, 6, 8 and 10 weeks after opening were compared among the three disinfectants. Results The optimal amounts of foam, gel and spray disinfectants were 0.7, 3.0 and 4.0 mL when the average kill log value was ≥1.00. The ethanol content of the foam disinfectant was more stable and the validity period was up to 10 weeks, while the validity periods of the gel disinfectant and spray disinfectant were 6 and 5 weeks respectively after opening. Conclusion The foam wash-free disinfectant achieves wash-free disinfection at 0.7 mL, and the shaped pump head structure of the bottle maintains active ingredient stability for more than 2 months after opening.