Abstract: Objective To analyze the effects of general anesthesia combined with epidural anesthesia on brain-gut peptide, intestinal barrier function and oxidative stress in patients with colorectal cancer. Methods Eighty-six patients with colorectal cancer who undergoing radical surgery in our hospital were enrolled as study objects, and were confirmed as colorectal cancer by pathological examination. Patients were divided into two groups by random number table method, among whom 43 cases with general anesthesia were selected as control group, and another 43 patients combining general anesthesia and epidural anesthesia were selected as observation group. The brain intestinal peptide, intestinal barrier function and oxidative stress response index were compared between the two groups. Results There were no significant differences in motilin, gastrin and ghrelin between the two groups before operation(P>0.05). The levels of motilin and ghrelin after operation in the observation group were significantly higher than those in the control group(P<0.05), but no significant difference in gastrin was observed between the two groups(P>0.05). There were no significant differences in serum diamine oxidase, D-lactate and endotoxin between the two groups before operation(P>0.05). After treatment, the above indicators in the two groups decreased significantly, but the control group decreased more significantly(P<0.05). After treatment, the superoxide dismutase - (SOD), glutathione(GSH)of the two groups were significantly increased, but the control group increased more significantly(P<0.05). After treatment, the serum malondialdehyde(MDA)level of the two groups showed a significant decrease trend, but the control group decreased more significantly(P<0.05). Conclusion Compared with general anesthesia alone, general anesthesia combined with epidural anesthesia can reduce the effects on brain gut peptide, intestinal barrier function and oxidative stress in colorectal cancer patients.