Abstract: Objective To explore the effect of TAT-modified short peptide LVKEI(TAT-LVKEI)on the proliferation and apoptosis of melanoma B16 cells. Methods The CCK-8 kit was used to detect cell proliferation viability, flow cytometry was used to detect cell cycle and cell apoptosis, and Western-blotting was used to detect apoptosis-related protein expression. Results TAT-LVKEI was able to inhibit the proliferation viability of melanoma cell line B16 cells and arrest cell cycle at G₀/G₁ phase. TAT-LVKEI was able to induce apoptosis of melanoma cell line B16 cells. TAT-LVKEI management was able to increase the expression levels of Cleaved Caspase-3 and Bax, and decrease expression levels of Bcl2 and Ki-67. Conclusion TAT-LVKEI can inhibit proliferation and promote apoptosis of melanoma B16 cells.