Abstract: Objective To observe the influence of the "One disease, One product" project on pregnancy outcomes and quality of life in patients with gestational diabetes mellitus(GDM). Methods A total of 124 GDM patients admitted to our hospital from June to December 2018 were selected as control group, and 166 GDM patients from January to June 2019 were selected as study group. The control group was given routine blood glucose control and nursing intervention, the study group implemented “One disease, One product” based on the control group. The rate of blood glucose reaching to normal standard value, pregnancy outcome, incidence of neonatal complications, breastfeeding rate on postpartum 42 days, quality of life score, and satisfaction with nursing services were compared between the two groups. Results Compared with the control group, the rate of blood glucose reaching to normal standard value and breastfeeding rate on postpartum 42 days in the study group were significantly higher(P<0.05), and the incidence of cesarean section, premature delivery, polyhydramnios, hypertension syndrome, fetal macrosomia, and newborn complications in the study group were significantly lower(P<0.05). The scores of quality of life on postpartum 42 days and satisfaction to nursing service in the study group were significantly higher than that in the control group(P<0.05). Conclusion “One disease, One product” project can effectively control blood glucose of GDM pregnant women, improve the maternal breastfeeding rate and postpartum quality of life, and these patients have higher care satisfaction.