Abstract: Objective To compare clinical value of the minimum inhibitory concentration(MIC)of microliquid culture medium and Roche's proportional method in the drug sensitivity test of mycobacterium tuberculosis. Methods A total of 100 mycobacterium tuberculosis strains samples provided by National Tuberculosis Laboratory of Liaoning Province were selected. The drug sensitivities of mycobacterium tuberculosis were tested by MIC and Roche's proportional method of microliquid culture medium. Results There were no significant differences in sensitivity of mycobacterium tuberculosis to drugs of ethambutol, dikanamycin, rifampicin, isoniazid, ofloxacin, and capreomycin detected by MIC method and Roche's proportional method(P>0.05). Conclusion The proportional method and MIC method have good consistency in the detection of drug sensitivity of mycobacterium tuberculosis, and has rapid detection and is economical. Therefore, they can provide theoretical basis for clinical treatmentof tuberculosis.