Expression of uc.189 exosomes in cervical carcinoma and its clinicopathological significance
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摘要:目的 检测uc.189在宫颈鳞癌中的RNA表达水平，观察uc.189外泌体的数量及其与临床的病理意义。方法 应用实时荧光定量PCR（qRT-PCR）检测uc.189在58例宫颈鳞癌和癌旁正常组织中的RNA表达水平，电镜下观察宫颈鳞癌组织中uc.189外泌体的数量，并分析其与宫颈鳞癌患者病理参数的相关性。结果 uc.189在宫颈鳞癌组织中的表达水平高于癌旁正常组织，差异有统计学意义（P < 0.05）。肿瘤组织中高含量的uc.189外泌体与宫颈鳞癌的分化程度、淋巴结转移和TNM分期均密切相关（P < 0.05）。结论 uc.189可能是一种宫颈癌的新促癌基因，其可通过外泌体形式参与宫颈鳞癌的发生、发展和转移过程。Abstract:Objective To detect the RNA expression level of uc.189 in cervical squamous cell carcinoma, and to observe the number of exosomes of uc.189 as well as its clinicopathological significance.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of uc.189 RNA in 58 cases of cervical squamous cell carcinoma and adjacent normal tissues. The number of uc.189 exosomes in cervical squamous cell carcinoma was observed under electron microscope, and the correlation between the number of uc.189 exosomes and pathological parameters of cervical squamous cell carcinoma was analyzed.Results The expression level of uc.189 in the cervical squamous cell carcinoma was significantly higher than that in the adjacent normal tissues (P < 0.05). The high number of uc.189 exosomes in tumor tissue was all closely related to the differentiation degree, lymph node metastasis and TNM stage of cervical squamous cell carcinoma (P < 0.05).Conclusion The uc.189 may be a new oncogene of cervical cancer. The uc.189 promotes the occurrence, development and metastasis of cervical squamous cell carcinoma through exosome.
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图 1 uc.189 RNA在宫颈鳞癌组织中的表达情况
A: uc.189 RNA在宫颈组织中的表达。与对照组相比, **P < 0.01。
B: uc.189 RNA在宫颈鳞癌转移组中的表达。与非转移组相比, *P < 0.05。![]()
图 3 uc.189外泌体的验证
A: 对照组和宫颈癌组uc.189 RNA表达水平图; B: Western blot检测外泌体标志物图。与对照组比较, *P < 0.05。
表 1 荧光定量引物信息表
引物名称 引物序列(5′-3′) uc.189 F: GATGGTTGTACTGATGGC R: TGGTCACATGACGCTGAA U6 F: CGCTTCGGCAGCACATATAC R: TTCACGAATTTGCGTGTCAT 
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表 2 宫颈鳞癌组织中uc.189外泌体的含量及其意义(x±s)
临床病理参数 高含量组
(n=39)低含量组
(n=19)χ2 P值 患者年龄 ＜55岁 18 10 0.215 0.781 ≥55岁 21 9 HPV感染 有 15 5 0.834 0.397 无 24 14 肿瘤大小 ＜3 cm 12 11 1.988 0.252 ≥3 cm 27 8 分化程度 高~中 10 13 9.771 0.004 低 29 6 淋巴结转移 阳性 30 4 16.441 0.001 阴性 9 15 TNM分期 Ⅰ~Ⅱ 8 12 10.284 0.003 Ⅲ~Ⅳ 31 7
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