宫颈癌中uc.189的表达及其临床病理意义

邓玉霞; 颜云; 王成海; 於晓平; 卢丹

doi:10.7619/jcmp.20201871

宫颈癌中uc.189的表达及其临床病理意义

邓玉霞^{1, 2},
颜云³,
王成海³,
於晓平⁴,
卢丹^1, ,

1.
扬州大学临床医学院妇产科, 江苏扬州, 225009
2.
江苏省宝应县人民医院妇产科, 江苏宝应, 225800
3.
扬州大学医学院病理教研室, 江苏扬州, 225009
4.
扬州大学附属医院健康体检中心, 江苏扬州, 225009

基金项目: 国家自然科学基金面上项目（82072088）；2020年江苏省扬州市科技计划项目（YZ2020253）

详细信息

通讯作者:
卢丹, E-mail: jxq8826@sina.com.cn

中图分类号: R365;R737.33
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- PDF下载量: 8
出版历程
- 收稿日期: 2020-12-28
- 网络出版日期: 2021-04-08
- 发布日期: 2021-03-27

Expression of uc.189 exosomes in cervical carcinoma and its clinicopathological significance

1.
Department Gynecology and Obstetrics, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, 225009
2.
Department Gynecology and Obstetrics, Baoying County People's Hospital of Jiangsu Province, Baoying, Jiangsu, 225009
3.
Pathological Teaching and Research Office, Medical College of Yangzhou University, Yangzhou, Jiangsu, 225009
4.
Health Examination Center, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, 225009

摘要

摘要:
目的检测uc.189在宫颈鳞癌中的RNA表达水平，观察uc.189外泌体的数量及其与临床的病理意义。
方法应用实时荧光定量PCR（qRT-PCR）检测uc.189在58例宫颈鳞癌和癌旁正常组织中的RNA表达水平，电镜下观察宫颈鳞癌组织中uc.189外泌体的数量，并分析其与宫颈鳞癌患者病理参数的相关性。
结果 uc.189在宫颈鳞癌组织中的表达水平高于癌旁正常组织，差异有统计学意义（P < 0.05）。肿瘤组织中高含量的uc.189外泌体与宫颈鳞癌的分化程度、淋巴结转移和TNM分期均密切相关（P < 0.05）。
结论 uc.189可能是一种宫颈癌的新促癌基因，其可通过外泌体形式参与宫颈鳞癌的发生、发展和转移过程。
- uc.189 /
- 外泌体 /
- 宫颈鳞癌 /
- 临床病理意义 /
- 淋巴结转移
Abstract:
Objective To detect the RNA expression level of uc.189 in cervical squamous cell carcinoma, and to observe the number of exosomes of uc.189 as well as its clinicopathological significance.
Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of uc.189 RNA in 58 cases of cervical squamous cell carcinoma and adjacent normal tissues. The number of uc.189 exosomes in cervical squamous cell carcinoma was observed under electron microscope, and the correlation between the number of uc.189 exosomes and pathological parameters of cervical squamous cell carcinoma was analyzed.
Results The expression level of uc.189 in the cervical squamous cell carcinoma was significantly higher than that in the adjacent normal tissues (P < 0.05). The high number of uc.189 exosomes in tumor tissue was all closely related to the differentiation degree, lymph node metastasis and TNM stage of cervical squamous cell carcinoma (P < 0.05).
Conclusion The uc.189 may be a new oncogene of cervical cancer. The uc.189 promotes the occurrence, development and metastasis of cervical squamous cell carcinoma through exosome.
- uc.189 /
- exosome /
- cervical squamous carcinoma /
- clinicopathologic significance /
- lymphatic metastasis

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图 1 uc.189 RNA在宫颈鳞癌组织中的表达情况

A: uc.189 RNA在宫颈组织中的表达。与对照组相比, **P < 0.01。
B: uc.189 RNA在宫颈鳞癌转移组中的表达。与非转移组相比, *P < 0.05。

下载: 全尺寸图片幻灯片

图 2 宫颈癌中uc.189外泌体形态和数量电镜图

A: 外泌体形态图(放大倍数250万倍); B: 外泌体数量图。与对照组比较, **P < 0.01。

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图 3 uc.189外泌体的验证

A: 对照组和宫颈癌组uc.189 RNA表达水平图; B: Western blot检测外泌体标志物图。与对照组比较, *P < 0.05。

下载: 全尺寸图片幻灯片

表 1 荧光定量引物信息表

引物名称	引物序列(5′-3′)
uc.189	F: GATGGTTGTACTGATGGC
	R: TGGTCACATGACGCTGAA
U6	F: CGCTTCGGCAGCACATATAC
	R: TTCACGAATTTGCGTGTCAT

下载: 导出CSV

表 2 宫颈鳞癌组织中uc.189外泌体的含量及其意义(x±s)

临床病理参数		高含量组 (n=39)	低含量组 (n=19)	χ²	P值
患者年龄	＜55岁	18	10	0.215	0.781
	≥55岁	21	9
HPV感染	有	15	5	0.834	0.397
	无	24	14
肿瘤大小	＜3 cm	12	11	1.988	0.252
	≥3 cm	27	8
分化程度	高~中	10	13	9.771	0.004
	低	29	6
淋巴结转移	阳性	30	4	16.441	0.001
	阴性	9	15
TNM分期	Ⅰ~Ⅱ	8	12	10.284	0.003
	Ⅲ~Ⅳ	31	7

下载: 导出CSV

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