Abstract: Objective To observe the distribution of pathogenic bacteria and drug sensitivity of patients with urogenital tract infection. Methods A total of 600 patients with urogenital tract infection were selected as study subjects, and urethral discharge of male patients and cervical secretions of female patients were collected, pathogen culture and drug sensitivity test were conducted in a sterile environment, the distribution of pathogenic bacteria and drug resistance were observed and analyzed. Results A total of 1 562 strains of pathogenic bacteria were isolated from 600 patients with urogenital tract infection, including 1 103 strains of Mycoplasma and 459 strains. Ureaplasma urealyticum(Uu)and Mycoplasma hominis(Mh)were main Mycoplasma infection, accounting for 33.03%(516/1 562)and 14.40%(225/1 562), respectively; Enterobacteriaceae and Enterococcus were the main bacteria, accounting for 11.65%(182/1 562)and 8.58%(134/1 562), respectively. The susceptibilities test showed that Uu and Mh were highly sensitive to josamycin, doxycycline, and minocycline, while their sensitivities to erythromycin and ofloxacin were low. Enterobacteriaceae was highly sensitive to imipenem, meroppenem, piperacillin/tazobactam, while was lowly sensitive to amoxicillin and ticacillin. Enterococcus was highly sensitive to vancomycin and tekoranin, while was lowly sensitive to erythromycin. Conclusion Mycoplasma infection is the main infection source in patients with urogenital tract infection, and bacteria infection is also presented. Therefore, clinical trials should be strengthened the susceptibility results of pathogenic bacteria in infected patients, so as to reasonably select antibacterial drugs.