Abstract: Objective To observe clinical effect of laparoscopic and choledochoscopic cholecystolithotomy in the treatment of cholecystolithiasis in children and adolescents. Methods The clinical data of 25 children and adolescents with cholecystolithiasis treated by laparoscopic and choledochoscopic gallbladder-preserving cholecystolithotomy under direct vision was retrospectively analyzed, and the clinical therapeutic effect was observed. Results All the patients, without severe complications, successfully completed minimally invasive gallbladder-preserving cholecystolithotomy, recovered well after operation, and maintained normal biochemical indicators. The postoperative biliarycolic symptoms of the patients were significantly improved, and B-ultrasound re-examination within 1 week after the operation showed no residual stones. The length of stay was 4 to 7 days, with an average of 5 days, and incision was healed well. All patients were followed up for 6 to 12 months, there was one patient with recurrence after 1 year of operation and laparoscopic cholecystectomy was conducted. Conclusion The combination of the laparoscopic and choledochoscopic minimally invasive gallbladder-preserving cholecystolithotomy is a safe and feasible for children and adolescents with cholecystolithiasis treatment.