Expression of long non-coding RNA cancer susceptibility candidate 11 in ovarian cancer tissues and its significance
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摘要:目的 观察长链非编码RNA(lncRNA)肿瘤易感候选基因11(CASC11)在卵巢癌组织中的表达情况，探讨抑制该基因对卵巢癌SKOV3细胞增殖、迁移和侵袭力的影响。方法 采用实时荧光定量聚合酶链反应(PCR)法检测卵巢癌组织和癌旁组织中CASC11表达情况。将SKOV3细胞分为si-CASC11组、阴性对照组和空白组，分别转染CASC11的干扰物序列、阴性对照序列和加入转染试剂，采用实时荧光定量PCR法检测细胞中CASC11表达情况，采用噻唑蓝(MTT)法检测细胞增殖活力，采用划痕实验检测细胞迁移能力，采用Transwell小室检测细胞侵袭能力。结果 卵巢癌组织中CASC11相对表达量高于癌旁组织，差异有统计学意义(P<0.001)。不同分化程度、国际妇产科联盟(FIGO)分期和淋巴结转移情况下，卵巢癌组织中CASC11相对表达量差异有统计学意义(P<0.05)。与阴性对照组和空白组比较, si-CASC11组细胞中CASC11相对表达量降低，细胞增殖活力、迁移能力、侵袭能力降低，差异有统计学意义(P<0.05或P<0.01)。结论 卵巢癌组织中CASC11呈高表达，且与恶性进展临床病理指标相关，抑制CASC11表达能够显著抑制SKOV3细胞增殖、迁移和侵袭能力。
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关键词:
- 卵巢癌 /
- 长链非编码RNA /
- 肿瘤易感候选基因11 /
- 细胞增殖 /
- 细胞侵袭
Abstract:Objective To observe the expression of long non-coding RNA (lncRNA) cancer susceptibility candidate 11 (CASC11) in tissues of ovarian cancer, and to explore the effects of CASC11 gene on cellular proliferation, migration and invasive ability of SKOV3 cells.Methods Real time fluorescent quantitative polymerase chain reaction (PCR) was used to detect the expressions of CASC11 gene in the tissues of ovarian cancer and adjacent tissues. The SKOV3 cells were divided into si-CASC11 group, negative control group and blank group, given interference sequence transfected by CASC11, negative control sequence and only transfection reagent only, respectively. The real time fluorescent quantitative PCR was used to detect the expression of CASC11 gene in the cells, and methyl tetrazolium (MTT) assay was used to detect cellular proliferation viability, scarification test was used to detect migration ability of the cells, and the Transwell chamber was used to detect invasive ability of cells.Results Compared with the adjacent tissues, the relative expression level of CASC11 in ovarian cancer tissues was significantly higher (P<0.001). The relative expression of CASC11 showed significant difference in terms of degree of differentiation, Federation International of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis (P<0.05). Compared with the negative control group and the blank group, the relative expression level of CASC11, the cell proliferation, migration, and invasion capabilities in the cells of si-CASC11 group were significantly reduced (P<0.05 or P<0.01).Conclusion The expression of CASC11 gene in ovarian cancer tissues is increased, and it is associated with the clinical pathological indicators of malignant progression. Inhibition of CASC11 expression can significantly inhibit the proliferation, migration and invasion of SKOV3 cell. -
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表 1 卵巢癌组织中CASC11表达与临床病理指标间的关系(x±s)
指标 n CASC11相对表达量 t P 年龄 ≥50岁 58 1.87±0.14 0.845 0.400 <50岁 46 1.90±0.14 组织学类型 浆液性 61 1.90±0.14 1.329 0.187 其他 43 1.86±0.13 分化程度 中高分化 67 1.86±0.13 2.212 0.029 低分化 37 1.93±0.16 FIGO分期 Ⅰ~Ⅱ期 48 1.85±0.12 2.653 0.009 Ⅲ~Ⅳ期 56 1.91±0.15 淋巴结转移 是 48 1.92±0.14 2.640 0.010 否 56 1.85±0.13 腹腔积液 是 59 1.87±0.14 1.053 0.295 否 45 1.90±0.14 FIGO: 国际妇产科联盟; CASC11: 肿瘤易感候选基因11。 
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表 2 3组细胞不同时点吸光度值比较(x±s)
时点 si-CASC11组 阴性对照组 空白组 12 h 0.21±0.08 0.16±0.04 0.20±0.06 24 h 0.23±0.11*# 0.47±0.11 0.44±0.15 48 h 0.41±0.07*# 0.58±0.12 0.57±0.09 72 h 0.49±0.12*# 0.67±0.11 0.66±0.05 96 h 0.58±0.13*# 0.87±0.07 0.79±0.11 与空白组比较, *P<0.05; 与阴性对照组比较, #P<0.05。
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表 3 3组细胞划痕愈合率比较(x±s)
% 组别 24 h 48 h si-CASC11组 13.50±3.02*# 29.83±1.33*# 阴性对照组 21.83±2.64 40.17±5.67 空白组 26.17±5.12 44.83±3.66 与空白组比较, *P<0.05; 与阴性对照组比较, #P<0.05。
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