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乳腺癌组织微小核糖核酸-485-3p、Xklp2靶蛋白表达水平及临床意义

Expression levels of microRNA-485-3p and Xklp2 target protein in breast cancer tissues and their clinical significance

    摘要
  • 摘要:
      目的  探讨乳腺癌组织微小核糖核酸-485-3p（miR-485-3p）、Xklp2靶蛋白（TPX2）表达水平与患者预后的关系。
      方法  采用免疫组织化学法检测TPX2蛋白表达，采用实时荧光定聚合酶链反应检测miR-485-3p、TPX2 mRNA表达。采用Pearson检验分析乳腺癌组织中miR-485-3p表达与TPX2 mRNA表达的关系；随访5年，采用Kaplan-Meier法分析乳腺癌组织中miR-485-3p、TPX2蛋白表达与患者总生存率的关系，并用COX回归分析影响乳腺癌患者总生存的危险因素。
      结果  乳腺癌组织中TPX2蛋白阳性率、TPX2 mRNA表达水平均高于癌旁正常组织，miR-485-3p表达水平低于癌旁正常组织，差异有统计学意义（P＜0.05）。乳腺癌组织中miR-485-3p、TPX2蛋白表达与患者TNM分期、淋巴结转移、Ki-67表达有关（P＜0.05）。乳腺癌组织中miR-485-3p表达与TPX2 mRNA表达呈负相关（P＜0.05）。miR-485-3p高表达者5年总生存率为77.78%，高于低表达者的52.94%，差异有统计学意义（P＜0.05）；TPX2蛋白阴性者5年总生存率为84.00%，高于阳性者的60.00%，差异有统计学意义（P＜0.05）。有淋巴结转移、miR-485-3p低表达、TPX2蛋白阳性是影响乳腺癌患者总生存的独立危险因素（P＜0.05）。
      结论  在乳腺癌组织中，miR-485-3p低表达、TPX2高表达及二者呈负相关，均可作为判断乳腺癌患者的预后指标。

     

    Abstract:
      Objective  To investigate the relationship between the expression levels of microRNA-485-3p (miR-485-3p) and Xklp2 target protein (TPX2) in breast cancer tissues and prognosis of patients.
      Methods  Immunohistochemistry was used to detect TPX2 protein expression, real-time fluorescent quantitative polymerase chain reaction was used to detect the expression of miR-485-3p and TPX2 mRNA. Pearson test was used to analyze the relationship between miR-485-3p expression and TPX2 mRNA expression in breast cancer tissues. Followed up for 5 years, the Kaplan-Meier method was used to analyze the relationships between the expressions of miR-485-3p, TPX2 proteins in breast cancer tissue and the overall survival rate of the patient, and COX regression analysis was used to analyze the risk factors affecting the overall survival of breast cancer patients.
      Results  The positive rate of TPX2 protein and the level of TPX2 mRNA expression in breast cancer tissues were significantly higher than those in normal tissues adjacent to cancer, the expression level of miR-485-3p was significantly lower than that of normal tissues adjacent to cancer (P < 0.05). The expression of miR-485-3p and TPX2 proteins in breast cancer tissues were related to TNM stage, lymph node metastasis and Ki-67 expression (P < 0.05). The expression of miR-485-3p in breast cancer tissue was negatively correlated with the expression of TPX2 mRNA (P < 0.05). The 5-year overall survival rate of patients with high miR-485-3p expression was 77.78%, which was significantly higher than 52.94% of patients with low miR-485-3p expression (P < 0.05); the 5-year overall survival rate of TPX2 negative patients was 84.00%, which was significantly higher than 60.00% of positive patients (P < 0.05). The lymph node metastasis, low expression of miR-485-3p, and positive TPX2 protein were independent risk factors affecting the overall survival of breast cancer patients (P < 0.05).
      Conclusion  In breast cancer tissues, miR-485-3p is lowly expressed and TPX2 is highly expressed. They are negatively correlated, and both can be used as prognostic indicators for judging breast cancer patients.

     

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