Expressions and significance of CXC chemokine ligand 12 and human cartilage glycoprotein-39 in serum and placental tissues of patients with preeclampsia
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摘要:目的 分析正常孕妇和子痫前期(PE)孕妇血清和胎盘组织中CXC型趋化因子配体12(CXCL12)和人类软骨糖蛋白-39(YKL-40)的表达。方法 采用酶联免疫吸附法(ELISA)测定50例正常妊娠孕妇(对照组)和50例PE孕妇(PE组)血清CXCL12、YKL-40浓度，采用免疫组化(IHC)两步法检测胎盘组织中CXCL12、YKL-40的蛋白表达水平。结果 PE组收缩压、舒张压、24 h尿蛋白定量高于对照组，新生儿出生体质量低于对照组，差异有统计学意义(P<0.05)。PE组患者血清CXCL12、YKL-40水平高于对照组，差异均有统计学意义(P<0.05)。IHC发现, PE组胎盘组织中CXCL12、YKL-40的表达强度均高于对照组，差异有统计学意义(P<0.05)。结论 PE患者CXCL12与YKL-40均高表达，提示PE的发病与血管内皮病变和炎症刺激有关。
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关键词:
- 子痫前期 /
- 胎盘 /
- CXC型趋化因子配体12 /
- 人类软骨糖蛋白-39 /
- 尿蛋白定量
Abstract:Objective To analyze the expressions of CXC chemokine ligand 12 (CXCL12) and human cartilage glycoprotein-39 (YKL-40) in serum and placental tissues of normal pregnant women and pregnant women with preeclampsia (PE).Methods The serum concentrations of CXCL12 and YKL-40 in 50 normal pregnant women (control group) and 50 pregnant women with PE (PE group) were measured by enzyme-linked immunosorbent assay (ELISA), and the protein expression levels of CXCL12 and YKL-40 in placenta were detected by two-step immunohistochemistry (IHC).Results The systolic blood pressure, diastolic blood pressure and 24-hour urinary protein quantification in the PE group were significantly higher than those in the control group, while the birth weight of newborns was significantly lower than that in the control group (P<0.05). The levels of serum CXCL12 and YKL-40 in the PE group were significantly higher than those in the control group (P<0.05). IHC found that the expression intensities of CXCL12 and YKL-40 in placental tissues of the PE group were significantly higher than those of the control group (P<0.05).Conclusion CXCL12 and YKL-40 are highly expressed in PE patients, which suggests that the pathogenesis of PE is related to vascular endothelial lesions and inflammatory stimulation. -
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表 1 2组孕妇一般资料比较(x±s)
一般资料 对照组(n=50) PE组(n=50) 年龄/岁 26.45±3.10 28.98±3.38 孕周/周 38.30±0.79 35.67±3.51 体质量指数/(kg/m2) 27.36±3.12 29.65±2.13 收缩压/mmHg 116.30±5.78 151.92±9.64* 舒张压/mmHg 76.10±6.83 108.90±9.25* 24 h尿蛋白定量/g 0.15±0.05 3.11±2.58* 新生儿出生体质量/g 3 209.20±309.06 2 394.28±312.38* 与对照组比较, * P<0.05。 
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表 2 2组血清CXCL12表达比较(x±s)
ng/mL 组别 n CXCL12 YKL-40 对照组 50 8.43±4.20 88.20±21.40 PE组 50 24.70±5.76* 119.52±46.38* CXCL12: CXC型趋化因子配体12;
YKL-40: 人类软骨糖蛋白-39。与对照组比较, * P<0.05。
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表 3 2组胎盘组织中CXCL12蛋白表达水平比较
组别 n CXCL12表达强度 χ2 P - + 
对照组 50 31 12 7 0 5.367 0.005 PE组 50 7 10 17 16
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表 4 2组胎盘组织中YKL-40蛋白表达水平比较
组别 n YKL-40表达强度 χ2 P - + 对照组 50 24 17 9 0 6.472 0.005 PE组 50 4 8 18 20
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