Correlation between single nucleotide polymorphism rs1333049 and susceptibility to coronary heart disease of Yi ethnic people in frontier plateau area
-
摘要:目的
探讨边疆高原地区世居彝族人群rs1333049位点单核苷酸多态性(SNP)与冠心病易感性的关系。
方法采用随机数表法选取在红河州滇南中心医院住院的边疆高原地区世居彝族患者120例作为研究对象，根据临床诊断结果分为冠心病组和对照组，每组60例。采集患者外周静脉血，采用TaqMan实时荧光定量聚合酶链反应(PCR)检测染色体9p21.3区域rs1333049位点基因分型。比较冠心病组和对照组的基因型(CC、CG、GG)频率、等位基因(C、G)频率和基因量，分析不同疾病阶段冠心病患者rs1333049基因型频率、等位基因频率和基因量，采用多因素Logistic回归分析探讨冠心病患者rs1333049 CG基因表达的影响因素。
结果冠心病组rs1333049 SNP位点CG基因型频率、CG基因量均高于对照组，差异有统计学意义(P < 0.05); 冠心病组中的急性心肌梗死患者rs1333049 SNP位点CG基因型频率、CG基因量均高于对照组，差异有统计学意义(P < 0.05); 冠心病组中的不稳定性心绞痛患者、稳定性心绞痛患者rs1333049 SNP位点的基因型频率、等位基因频率及基因量与对照组比较，差异均无统计学意义(P>0.05)。边疆高原地区世居彝族冠心病患者的rs1333049位点CG基因量表达与左主干(LM)/左前降支(LAD)病变有关(P < 0.05), 与血脂指标、空腹血糖(FBG)、冠心病不同疾病阶段、右冠状动脉病变、左回旋支病变及各支冠状动脉狭窄程度(Gensini积分)无关(P>0.05)。多因素Logistic回归分析显示, FBG(OR=1.55, 95%CI为1.01~2.36)、LM/LAD病变(OR=1.87, 95%CI为1.10~3.16)是边疆高原地区彝族冠心病患者rs1333049 CG基因表达的独立影响因素(P < 0.05)。
结论rs1333049 CG基因为边疆高原地区世居彝族人群的冠心病易感基因，而FBG水平、LM/LAD病变是rs1333049 CG基因表达的影响因素。
Abstract:ObjectiveTo explore the relationship between single nucleotide polymorphism(SNP) rs1333049 and susceptibility to coronary heart disease of permanently inhabited Yi ethnic people in frontier plateau area.
MethodsUsing the random number table method, 120 cases of permanently inhabited Yi ethnic patients of frontier plateau area in Diannan Central Hospital of Honghe Prefecture were selected as study objects, and were divided into coronary artery disease group and control group according to clinical diagnosis results, with 60 cases in each group. Peripheral venous blood was collected and rs1333049 genotype in 9p21.3 region of chromosomewas detected by TaqMan real-time fluorescence quantitative polymerase chain reaction (PCR). The frequency of genotypes (CC, CG, GG), frequency of allele (C, G) and gene amount were compared between the coronary artery disease group and the control group. The genotype frequency, allele frequency and gene amount of rs1333049 in coronary artery disease patients with different disease stages were analyzed. MultivariateLogistic regression analysis was used to explore the influencing factors of rs1333049 CG gene expression in patients with coronary heart disease.
ResultsThe CG genotype frequency and CG gene amount of rs1333049 SNP locus in the coronary artery disease group were higher than those in the control group (P < 0.05). The CG genotype frequency and CG gene amount of rs1333049 SNP locus in patients with acute myocardial infarction in the coronary heart disease group were higher than those in the control group (P < 0.05). There were no significant differences in genotype frequency, allele frequency and gene amount of rs1333049 SNP locus in patients with unstable angina pectoris and stable angina pectoris in the coronary heart disease group when compared with those of the control group (P>0.05). CG gene amount expression of rs1333049 was associated with left main branch (LM)/left anterior descending branch (LAD) lesions in permanently inhabited Yi ethnic patients with coronary artery disease in the border plateau area (P < 0.05), but had no correlations with blood lipid indexes, fasting blood glucose (FBG), different disease stages of coronary heart disease, right coronary artery disease, lesion of left circumflex branch and the stenosis degree of each coronary artery (Gensini score) (P>0.05). Multivariate Logistic regression analysis showed that FBG (OR=1.55; 95%CI, 1.01 to 2.36), LM/LAD lesions (OR=1.87; 95%CI, 1.10 to 3.16) were independent influencing factors of rs1333049 CG gene expression in Yi ethnic patients with coronary heart disease in border and plateau areas (P < 0.05).
ConclusionThe rs1333049 CG gene is a susceptibility gene for coronary heart disease in permanently inhabited Yi ethnic people in frontier plateau, while FBG and LM/LAD lesion are influencing factors for expression of rs1333049 CG gene.
-
-
表 1 2组rs1333049 SNP基因型频率、等位基因频率及基因量比较[n(%)]
指标 分类 对照组(n=60) 冠心病组(n=60) df χ2 P rs1333049基因型频率 CC 17(28.3) 9(15.0) 2 6.102 0.047 CG 22(36.7) 35(58.3) GG 21(35.0) 16(26.7) rs1333049等位基因频率 C 56(46.7) 53(44.2) 1 0.151 0.679 G 64(53.3) 67(55.8) rs1333049基因量 CC 17(28.3) 9(15.0) 1 3.142 0.076 CG+GG 43(71.7) 51(85.0) GG 21(35.0) 16(26.7) 1 0.977 0.323 CG+CC 39(65.0) 44(73.3) CG 22(36.7) 35(58.3) 1 5.647 0.017 CC+GG 38(63.3) 25(41.7) 
下载: 导出CSV
表 2 对照组和不同疾病阶段冠心病患者rs1333049基因型频率、等位基因频率及基因量比较[n(%)]
指标 分类 对照组(n=60) AMI组(n=25) UA组(n=15) SAP组(n=20) rs1333049基因型频率 CC 17(28.3) 3(12.0) 5(33.3) 1(5.0) CG 22(36.7) 17(68.0) 7(46.7) 11(55.0) GG 21(35.0) 5(20.0) 3(20.0) 8(40.0) df — 2 2 2 χ2 — 7.075 1.256 4.955 P — 0.029 0.534 0.084 rs1333049等位基因频率 C 56(46.7) 23(46.0) 17(56.7) 13(32.5) G 64(53.3) 27(54.0) 13(43.3) 27(67.5) df — 1 1 1 χ2 — 0.006 0.961 2.455 P — 0.937 0.327 0.117 rs1333049基因量 CG 22(36.7) 17(68.0) 7(46.7) 11(55.0) CC+GG 38(63.3) 8(32.0) 8(53.3) 9(45.0) df — 1 1 1 χ2 — 6.978 0.506 1.256 P — 0.008 0.477 0.534 AMI组、UA组、SAP组的df、χ2、P数值均为各组指标分别与对照组比较所得。
AMI: 急性心肌梗死; UA: 不稳定性心绞痛; SAP: 稳定性心绞痛。
下载: 导出CSV
表 3 冠心病患者rs1333049 SNP位点CG、GG+CC基因量的单因素分析(x±s)[n(%)]
因素 rs1333049基因量 χ2/t P CG(n=35) CC+GG(n=25) 年龄/岁 57.44±9.91 58.82±8.98 -0.419 0.677 LDL-C/(mmol/L) 2.73±0.95 3.03±0.94 -0.876 0.384 TG/(mmol/L) 1.85±1.40 2.35±0.25 -0.451 0.654 HDL-C/(mmol/L) 1.10±0.37 1.11±0.33 -0.094 0.926 FBG/(mmol/L) 6.65±2.35 6.40±1.97 0.342 0.733 TC/(mmol/L) 4.62±1.20 4.63±0.47 -0.011 0.991 AMI 17(68.0) 8(32.0) 1.893 0.388 UA 7(46.7) 8(53.3) SAP 11(55.0) 9(45.0) LM/LAD病变 34(63.0) 20(37.0) 4.762 0.029 RCA病变 27(60.0) 18(40.0) 0.206 0.650 LCX病变 19(57.6) 14(42.4) 0.017 0.895 LM+LAD的Gensini积分/分 24.20±28.94 18.96±21.89 0.762 0.449 RCA的Gensini积分/分 9.80±12.77 10.3±14.15 -0.149 0.882 LCX的Gensini积分/分 6.07±9.48 7.56±15.29 -0.465 0.644 各血管总Gensini积分/分 40.07±36.50 36.84±34.40 0.346 0.730 LDL-C: 低密度脂蛋白胆固醇; TG: 甘油三酯; HDL-C: 高密度脂蛋白胆固醇; FBG: 空腹血糖; TC: 总胆固醇; AMI: 急性心肌梗死; UA: 不稳定性心绞痛; SAP: 稳定性心绞痛; LM: 左主干; LAD: 左前降支; RCA: 右冠状动脉; LCX: 左回旋支。
下载: 导出CSV
表 4 冠心病患者易感基因rs1333049 CG基因的多因素Logistic回归分析
因素 B SE Wald OR 95%CI P 截距 9.22 3.63 6.45 — — 0.011 年龄 0.02 0.04 0.22 1.02 0.94~1.10 0.636 LDL-C 0.03 0.54 < 0.01 1.03 0.36~2.96 0.957 TG 0.15 0.27 0.33 0.86 0.51~1.45 0.568 HDL-C 1.44 1.00 2.09 4.23 0.60~29.82 0.148 FBG 0.44 0.22 4.02 1.55 1.01~2.36 0.045 TC 0.77 0.48 2.64 2.17 0.85~5.52 0.104 SAP — — — 1 — — AMI 1.24 0.77 2.59 0.29 0.06~1.31 0.108 UA 0.62 1.00 0.39 0.54 0.08~3.80 0.534 LM/LAD病变 0.61 0.27 5.39 1.87 1.10~3.16 0.020 RCA病变 0.40 0.77 0.28 1.50 0.33~6.73 0.600 LCX病变 0.61 0.85 0.51 1.84 0.35~9.78 0.474 LDL-C: 低密度脂蛋白胆固醇; TG: 甘油三酯; HDL-C: 高密度脂蛋白胆固醇; FBG: 空腹血糖; TC: 总胆固醇;
SAP: 稳定性心绞痛; AMI: 急性心肌梗死; UA: 不稳定性心绞痛; LM: 左主干; LAD: 左前降支;
RCA: 右冠状动脉; LCX: 左回旋支。
下载: 导出CSV
-
[1] 刘斌, 李显刚, 张敏, 等. 影响冠心病患者二次住院的主要因素分析[J]. 昆明医科大学学报, 2021, 42(6): 103-109. [2] ZARKASI K A, ABDUL MURAD N A, AHMAD N, et al. Coronary heart disease in type 2 diabetes mellitus: genetic factors and their mechanisms, gene-gene, and gene-environment interactions in the Asian populations[J]. Int J Environ Res Public Heal, 2022, 19(2): 647. doi: 10.3390/ijerph19020647
[3] HU L N, SU G Y, WANG X. The roles of ANRIL polymorphisms in coronary artery disease: a meta-analysis[J]. Biosci Rep, 2019, 39(12): BSR20181559. doi: 10.1042/BSR20181559
[4] XIE Y L, ZHAO D, DONG P S, et al. Effects of ANRIL polymorphisms on the likelihood of coronary artery disease: a meta-analysis[J]. J Cell Biochem, 2019, 120(4): 6113-6119. doi: 10.1002/jcb.27898
[5] 中华医学会心血管病学分会, 中华心血管病杂志编辑委员会. 急性ST段抬高型心肌梗死诊断和治疗指南(2019)[J]. 中华心血管病杂志, 2019, 47(10): 766-767. [6] 中华医学会, 中华医学会杂志社, 中华医学会全科医学分会, 等. 非ST段抬高型急性冠状动脉综合征基层诊疗指南(2019年)[J]. 中华全科医师杂志, 2021, 20(1): 6-13. doi: 10.3760/cma.j.cn114798-20201030-01112 [7] 中华医学会心血管病学分会介入心脏病学组, 中华医学会心血管病学分会动脉粥样硬化与冠心病学组, 中国医师协会心血管内科医师分会血栓防治专业委员会, 等. 稳定性冠心病诊断与治疗指南[J]. 中华心血管病杂志, 2018, 46(9): 680-694. doi: 10.3760/cma.j.issn.0253-3758.2018.09.004 [8] SCHULTZ A, DAHL L, MCGIBBON E, et al. Differences in coronary artery disease complexity and associations with mortality and hospital admissions among First Nations and non-First Nations patients undergoing angiography: a comparative retrospective matched cohort study[J]. CMAJ Open, 2020, 8(4): E685-E694. doi: 10.9778/cmajo.20190171
[9] BUDOFF M J, YOUNG R, BURKE G, et al. Ten-year association of coronary artery calcium with atherosclerotic cardiovascular disease (ASCVD) events: the multi-ethnic study of atherosclerosis (MESA)[J]. Eur Heart J, 2018, 39(25): 2401-2408. doi: 10.1093/eurheartj/ehy217
[10] FABER J E, STORZ J F, CHEVIRON Z A, et al. High-altitude rodents have abundant collaterals that protect against tissue injury after cerebral, coronary and peripheral artery occlusion[J]. J Cereb Blood Flow Metab, 2021, 41(4): 731-744. doi: 10.1177/0271678X20942609
[11] MA Y L, ZHU L L, MA Z J, et al. Distinguishing feature of gut microbiota in Tibetan highland coronary artery disease patients and its link with diet[J]. Sci Rep, 2021, 11: 18486. doi: 10.1038/s41598-021-98075-9
[12] PATEL R S, SCHMIDT A F, TRAGANTE V, et al. Association of chromosome 9p21 with subsequent coronary heart disease events[J]. Circ Genom Precis Med, 2019, 12(4): e002471. doi: 10.1161/CIRCGEN.119.002471
[13] TAQUETI V R, DI CARLI M F. Coronary microvascular disease pathogenic mechanisms and therapeutic options: JACC state-of-the-art review[J]. J Am Coll Cardiol, 2018, 72(21): 2625-2641. doi: 10.1016/j.jacc.2018.09.042
[14] MORRIS P D, IQBAL J, CHIASTRA C, et al. Simultaneous kissing stents to treat unprotected left main stem coronary artery bifurcation disease; stent expansion, vessel injury, hemodynamics, tissue healing, restenosis, and repeat revascularization[J]. Catheter Cardiovasc Interv, 2018, 92(6): E381-E392. doi: 10.1002/ccd.27640
[15] FISHMAN S L, SONMEZ H, BASMAN C, et al. The role of advanced glycation end-products in the development of coronary artery disease in patients with and without diabetes mellitus: a review[J]. Mol Med, 2018, 24(1): 59. doi: 10.1186/s10020-018-0060-3
-
期刊类型引用(6)
1. 王姝琪. 以埃索美拉唑和奥美拉唑为主的三联疗法治疗幽门螺杆菌阳性胃溃疡的比较. 中国实用乡村医生杂志. 2022(04): 29-32 . 
百度学术
2. 齐义. 序贯药物方案与四联疗法对Hp阳性慢性胃炎活动期患者Hp根除率及复发的影响. 齐齐哈尔医学院学报. 2021(02): 127-129 . 百度学术
3. 殷久勇. 序贯疗法与四联疗法治疗幽门螺杆菌感染性胃溃疡的临床对照研究. 江西医药. 2021(10): 1735-1738 . 百度学术
4. 张曼，高炳霞，齐颖，张盈盈. 依卡倍特钠联合常规四联疗法对幽门螺杆菌阳性慢性萎缩性胃炎的疗效观察. 现代消化及介入诊疗. 2020(01): 76-79 . 百度学术
5. 刘小辉. 四联疗法对内镜黏膜下剥离术后幽门螺杆菌感染患者炎症因子水平及愈合质量的影响. 黑龙江医学. 2020(02): 210-212 . 百度学术
6. 钟文洲，林松挺，李春芸. 十天序贯疗法与标准三联疗法治疗幽门螺杆菌感染的疗效及对患者昼夜胃内p H的影响. 中国临床研究. 2019(01): 46-49 . 百度学术
其他类型引用(1)
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
- 被引次数: 7
苏公网安备 32100302010246号