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结直肠癌组织中高迁移率蛋白族1与血管内皮生长因子、微血管密度的相关性研究

Relationships of high mobility protein group 1 with vascular endothelial growth factor and microvessel density in colorectal cancer

    摘要
  • 摘要:
    目的 观察结直肠癌患者癌组织中高迁移率蛋白族1(HMGB1)、血管内皮生长因子(VEGF)和微血管密度(MVD)的表达情况，并探讨HMGB1与VEGF、MVD的相关性。
    方法 将89例结直肠癌患者根据是否发生淋巴结转移分为转移组(n=32)和未转移组(n=57), 检测2组癌组织中HMGB1、VEGF和MVD表达情况，利用受试者工作特征(ROC)曲线计算HMGB1、VEGF和MVD诊断结直肠癌患者淋巴结转移情况的最佳截断值，并分析HMGB1与VEGF、MVD的相关性，建立线性回归方程。
    结果 转移组癌组织与癌旁组织中的HMGB1阳性评分、VEGF阳性评分和MVD均高于未转移组，转移组、未转移组癌组织中的HMGB1阳性评分、VEGF阳性评分和MVD均高于癌旁组织，差异有统计学意义(P < 0.05)。ROC曲线显示，癌组织中HMGB1、VEGF和MVD诊断淋巴结转移的最佳截断值分别为阳性评分4.5分、阳性评分3.5分和平均每个200倍视野下32.5条。VEGF高表达组、MVD高表达组淋巴结转移者占比、HMGB1高表达者占比均分别高于VEGF低表达组、MVD低表达组，差异有统计学意义(P < 0.05)。癌组织中HMGB1阳性评分与VEGF阳性评分、MVD呈正相关，线性回归方程分别为yVEGF=2.459+0.516xHMGB1以及yMVD=-17.365+13.502xHMGB1。
    结论 结直肠癌患者癌组织中HMGB1、VEGF和MVD均呈现高表达状态，且HMGB1表达与VEGF、MVD具有相关性。临床医师可通过HMGB1检测
    结果 评估结直肠癌患者肿瘤部位血管新生情况，及时有效预防淋巴结转移。

     

    Abstract:
    Objective To observe the expressions of high mobility protein group 1 (HMGB1), vascular endothelial growth factor (VEGF) and microvessel density (MVD) in colorectal cancer tissues, and the correlations of HMGB1 with VEGF and MVD.
    Methods A total of 89 patients with colorectal cancer were divided into metastasis group (n=32) and non-metastasis group (n=57) according to whether lymph node metastasis occurred or not. The expressions of HMGB1, VEGF and MVD in cancer tissues of the two groups were detected. The best cut-off values of three indexes for the diagnosis of lymph node metastasis in patients with colorectal cancer were obtained by receiver operating characteristic (ROC) curve. The correlations of HMGB1 with VEGF and MVD were analyzed, and the linear regression equation was established.
    Results The positive scores of HMGB1 and VEGF and MVD in cancer tissues and adjacent tissues in the metastasis group were higher than those in the non-metastasis group, and the positive scores of HMGB1 and VEGF and the number of MVD in cancer tissues in the two groups were higher than those in adjacent tissues (P < 0.05). ROC curve results showed that the best cut-off values of HMGB1 and VEGF for diagnosis of lymph node metastasis in cancer tissues were 4.5 points and 3.5 points, respectively, and the best cut-off value of MVD was 32.5 per 200 times field of vision. The proportions of patients with lymph node metastasis and HMGB1 overexpression in the VEGF high expression group and MVD high expression group were higher than those in low expression groups for VEGF and MVD(P < 0.05). In cancer tissues, the positive score of HMGB1 was positively correlated with positive score of VEGF and MVD. The linear regression equation was established, yVEGF=2.459+0.516xHMGB1 and yMVD=-17.365+13.502xHMGB1.
    Conclusion HMGB1, VEGF and MVD are overexpressed in the cancer tissues of patients with colorectal cancer, and the expression of HMGB1 is significantly correlated with VEGF and MVD. The angiogenesis of tumor can be detected by HMGB1 and effective preventive measures for lymph node metastasis should be made in time.

     

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