Relationships of BTB/POZ domain-containing protein 7 and matrix metalloproteinase-9 expression with autoantibodies in labial gland tissues of patients with primary Sjögren's syndrome
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摘要:目的
探讨原发性干燥综合征(pSS)患者唇腺组织中BTB/POZ结构域蛋白7(BTBD7)及基质金属蛋白酶-9(MMP-9)的表达与自身抗体的关系。
方法收集2016年9月—2020年10月本院46例pSS患者的唇腺组织为pSS组，收集同期46例口干燥症患者的唇腺组织为对照组。检测唇腺组织中BTBD7、MMP-9的mRNA表达及蛋白表达; 分析pSS患者血清自身抗体[抗干燥综合征抗原A(SSA)抗体、抗干燥综合征抗原B(SSB)抗体、抗核抗体(ANA)]与BTBD7、MMP-9的关系; 分析BTBD7蛋白表达水平与MMP-9蛋白表达水平的相关性。
结果与对照组比较, pSS组患者唇腺组织中BTBD7、MMP-9的mRNA及蛋白表达水平较高，差异有统计学意义(P < 0.05)。在pSS患者的抗SSA抗体、抗SSB抗体以及ANA的检测结果中，各抗体阳性者唇腺组织中BTBD7、MMP-9的mRNA及蛋白表达水平均高于阴性者，差异均有统计学意义(P < 0.05)。相关性分析显示, pSS患者唇腺组织中BTBD7蛋白表达水平与MMP-9蛋白表达水平呈显著正相关(r=0.493, P < 0.05)。
结论pSS患者唇腺组织中BTBD7、MMP-9均呈高表达，且二者与自身抗体如抗SSA抗体、抗SSB抗体及ANA表达有密切联系。
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关键词:
- 原发性干燥综合征 /
- 唇腺组织 /
- BTB/POZ结构域蛋白7 /
- 基质金属蛋白酶-9 /
- 自身抗体
Abstract:ObjectiveTo investigate the relationships of BTB/POZ domain-containing protein 7 (BTBD7) and matrix metalloproteinase-9 (MMP-9) expression with autoantibodies in labial gland tissues of patients with primary SjÖgren′s syndrome (pSS).
MethodsFrom September 2016 to October 2020, the labial gland tissues of 46 patients with pSS were collected as pSS group, and the labial gland tissues of 46 patients with xerostomia in the same period were collected as control group. The mRNA and protein expressions of BTBD7 and MMP-9 in labial gland tissues were detected; the relationships of the serum autoantibodies [anti-SjÖgren′s syndrome antigen A (SSA) antibody, anti-SjÖgren′s syndrome antigen B (SSB) antibody, and antinuclear antibody (ANA)] with BTBD7 and MMP-9 were analyzed in patients with pSS; the correlation between protein expression level of BTBD7 and protein expression level of MMP-9 was analyzed.
ResultsCompared with the control group, the mRNA and protein expression levels of BTBD7 and MMP-9 in the labial gland tissues of patients in the pSS group were significantly higher (P < 0.05). In the detection results of anti-SSA antibody, anti-SSB antibody and ANA antibody in the patients with pSS, the mRNA and protein expression levels of BTBD7 and MMP-9 in the labial gland tissues of positive patients were significantly higher than those of negative patients (P < 0.05). The correlation analysis showed that there was a significant positive correlation between protein expression level of BTBD7 and protein expression level of MMP-9 in the labial gland tissues of patients with pSS (r=0.493, P < 0.05).
ConclusionBTBD7 and MMP-9 are highly expressed in the labial gland tissues of patients with pSS, and are closely related to the expressions of autoantibodies such as anti-SSA antibody, anti-SSB antibody and ANA.
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表 1 2组患者唇腺组织中BTBD7、MMP-9的mRNA及蛋白表达水平比较(x±s)
组别 n BTBD7 mRNA MMP-9 mRNA BTBD7/GAPDH MMP-9/GAPDH 对照组 46 1.00±0 1.00±0 0.34±0.05 0.27±0.06 pSS组 46 1.79±0.15* 2.14±0.16* 0.89±0.08* 0.93±0.12* BTBD7: BTB/POZ结构域蛋白7; MMP-9: 基质金属蛋白酶-9。与对照组比较, *P<0.05。 
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表 2 不同血清自身抗体结果的pSS患者唇腺组织中BTBD7、MMP-9的mRNA及蛋白表达水平比较(x±s)
抗体表达 n BTBD7 mRNA MMP-9 mRNA BTBD7/GAPDH MMP-9/GAPDH 抗SSA抗体阴性 22 1.59±0.12 1.98±0.14 0.82±0.07 0.85±0.09 抗SSA抗体阳性 24 1.97±0.13* 2.29±0.15* 0.96±0.11* 1.01±0.13* 抗SSB抗体阴性 34 1.72±0.15 2.05±0.15 0.84±0.08 0.89±0.11 抗SSB抗体阳性 12 1.98±0.10* 2.38±0.11* 1.05±0.10* 1.06±0.10* ANA阴性 7 1.54±0.09 1.88±0.10 0.64±0.06 0.70±0.08 ANA阳性 39 1.84±0.14* 2.19±0.14* 0.93±0.12* 0.97±0.13* SSA: 干燥综合征抗原A; SSB: 干燥综合征抗原B; ANA: 抗核抗体。与同种抗体阴性者比较, *P<0.05。
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