长链非编码RNA LINC02178对膀胱癌细胞增殖、侵袭及迁移的影响及机制研究

卢保德; 韦柳兴; 李镇杰; 林志弟; 黄勇平

doi:10.7619/jcmp.20223660

长链非编码RNA LINC02178对膀胱癌细胞增殖、侵袭及迁移的影响及机制研究

右江民族医学院附属医院泌尿外科, 广西百色, 533000

基金项目:

广西壮族自治区百色市科学研究与技术开发计划百科20213719

详细信息

通讯作者:
黄勇平, E-mail: h.y.p.1968@163.com

中图分类号: R737.14;Q522
计量
- 文章访问数: 154
- HTML全文浏览量: 43
- PDF下载量: 21
出版历程
- 收稿日期: 2022-12-07
- 修回日期: 2023-03-05
- 网络出版日期: 2023-06-06

Effects and mechanism of long non-coding RNA LINC02178 on proliferation, invasion and migration of bladder cancer cells

Department of Urological Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000

摘要

摘要:
目的
探讨长链非编码RNA LINC02178对膀胱癌细胞增殖、侵袭、迁移的影响及机制。
方法
基于公共数据库分析LINC02178在膀胱癌组织中的表达水平。采用逆转录-实时定量聚合酶链反应(RT-qPCR)检测LINC02178在膀胱癌细胞中的表达; 采用si-LINC02178、si-NC片段转染J82细胞; 采用噻唑蓝(MTT)实验和Transwell法检测沉默LINC02178后细胞增殖、侵袭和迁移能力的变化; 采用Western blot检测磷脂酰肌醇-3-激酶(PI3K)、磷酸化PI3K(p-PI3K)、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)蛋白的表达水平。
结果
LINC02178在膀胱癌组织中高表达，且高表达患者生存预后较低表达患者更差，差异有统计学意义(P < 0.05)。LINC02178在膀胱癌细胞J82、T24、5637中的表达水平高于膀胱正常上皮细胞，差异有统计学意义(P < 0.05)。si-LINC02178组细胞增殖能力弱于si-NC组，差异有统计学意义(P < 0.05)。si-LINC02178和si-NC组细胞侵袭数分别为(91.33±2.02)、(179.30±4.26)个，迁移数分别为(98.33±3.84)、(196.30±2.73)个，差异均有统计学意义(P < 0.05)。沉默LINC02178后，细胞中p-PI3K、p-Akt蛋白的表达下调，差异有统计学意义(P < 0.05)，而PI3K、Akt蛋白无显著变化(P>0.05)。
结论
LINC02178表达与膀胱癌患者预后不良有关，沉默LINC02178可抑制膀胱癌细胞增殖、侵袭和迁移，其机制可能是通过PI3K/Akt信号通路进行调控。
- 膀胱癌 /
- LINC02178 /
- 增殖 /
- 侵袭 /
- 迁移 /
- 磷脂酰肌醇-3-激酶 /
- 蛋白激酶B
Abstract:
Objective
To investigate the expression of long non-coding RNA LINC02178 in bladder cancer and its effects on the proliferation, invasion and migration of bladder cancer cells.
Methods
The expression level of LINC02178 in bladder cancer tissues was analyzed based on the public database. The expression of LINC02178 in bladder cancer cells was detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Si-LINC02178 and si-NC fragments were transfected into J82 cells, and the changes of proliferation, invasion and migration of the expression of LINC02178 after silencing were detected by Methyl Thiazolyl Tetrazolium (MTT) and Transwell methods. The protein expression levels of phosphatidylinositol-3-kinase (PI3K), phosphorylated PI3K(p-PI3K), protein kinase B(Akt), phosphorylated Akt(p-Akt) protein were detected by Western blot.
Results
LINC02178 was highly expressed in bladder cancer tissues, and the survival prognosis of patients with high expression was worse compared with those with low expression (P < 0.05). The expression of LINC02178 in bladder cancer cell lines J82, T24 and 5637 was significantly higher than that in normal bladder epithelial cells (P < 0.05). The cell proliferation ability of the si-LINC02178 group was lower than that of the si-NC group (P < 0.05). The number of cells invaded in the si-LINC02178 group and si-NC group was (91.33±2.02) and (179.30±4.26), and the number of cells migrated was (98.33±3.84) and (196.30±2.73), respectively, which showed statistically significant difference between the two groups (P < 0.05). After silencing LINC02178, the expression of p-PI3K and p-Akt protein in cells was down-regulated, while PI3K and Akt had no significant changes (P>0.05).
Conclusion
LINC02178 expression is associated with poor prognosis of patients with bladder cancer. Silencing LINC02178 expression can inhibit the proliferation, invasion and migration of bladder cancer cells, which may be regulated by the PI3K/Akt signaling pathway.
- bladder cancer /
- LINC02178 /
- proliferation /
- invasion /
- migration /
- phosphatidylinositol-3-kinase /
- protein kinase B

HTML全文

图 1 LINC02178在膀胱癌组织中的表达及与预后的关系

A: LINC02178在膀胱癌中的表达; B: LINC02178与膀胱癌预后的关系。

下载: 全尺寸图片幻灯片

图 2 LINC02178在膀胱癌细胞中的表达水平

与SV-HUC-1比较, ***P < 0.001。

下载: 全尺寸图片幻灯片

图 3 LINC02178在J82细胞敲低效率验证

与si-NC组比较, ***P < 0.001。

下载: 全尺寸图片幻灯片

图 4 沉默LINC02178后细胞增殖曲线

与si-LINC02178组比较, **P < 0.01, ***P < 0.001。

下载: 全尺寸图片幻灯片

图 5 沉默LINC02178后细胞侵袭能力变化

与si-NC组比较, ***P < 0.001。

下载: 全尺寸图片幻灯片

图 6 沉默LINC02178后细胞迁移能力变化

与si-NC组比较, ***P < 0.001。

下载: 全尺寸图片幻灯片

图 7 沉默LINC02178表达后的蛋白表达变化

与si-NC组比较, **P < 0.01, ***P < 0.001。

下载: 全尺寸图片幻灯片

表 1 LINC02178表达与临床病理特征关系

临床病理特征	分类	n	LINC02178低表达	LINC02178高表达	P
年龄	≤70岁	234	115	119	0.766
	>70岁	180	92	88
性别	女	109	63	46	0.074
	男	305	144	161
分级	低级别	21	11	10	0.991
	高级别	390	194	196
T分期	T1	5	5	0	0.008
	T2	119	67	52
	T3	196	95	101
	T4	60	22	38
淋巴结转移	无	239	122	117	0.095
	有	128	57	71
远处转移	无	202	104	98	0.936
	有	11	5	6

下载: 导出CSV

参考文献(20)

[1]	SUNG H, FERLAY J, SIEGEL R L, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249. doi: 10.3322/caac.21660
[2]	KOPP F, MENDELL J T. Functional classification and experimental dissection of long noncoding RNAs[J]. Cell, 2018, 172(3): 393-407. doi: 10.1016/j.cell.2018.01.011
[3]	SUN Z L, JING C Y, XIAO C T, et al. An autophagy-related long non-coding RNA prognostic signature accurately predicts survival outcomes in bladder urothelial carcinoma patients[J]. Aging, 2020, 12(15): 15624-15637. doi: 10.18632/aging.103718
[4]	WITJES J A, BRUINS H M, CATHOMAS R, et al. European association of urology guidelines on muscle-invasive and metastatic bladder cancer: summary of the 2020 guidelines[J]. Eur Urol, 2021, 79(1): 82-104. doi: 10.1016/j.eururo.2020.03.055
[5]	PATEL V G, OH W K, GALSKY M D. Treatment of muscle-invasive and advanced bladder cancer in 2020[J]. CA Cancer J Clin, 2020, 70(5): 404-423. doi: 10.3322/caac.21631
[6]	KIM I H, LEE H J. Perioperative systemic treatment for muscle-invasive bladder cancer: current evidence and future perspectives[J]. Int J Mol Sci, 2021, 22(13): 7201. doi: 10.3390/ijms22137201
[7]	SUNG H, SIEGEL R L, TORRE L A, et al. Global patterns in excess body weight and the associated cancer burden[J]. CA Cancer J Clin, 2019, 69(2): 88-112. doi: 10.3322/caac.21499
[8]	ZHANG X Y, XIE K, ZHOU H H, et al. Role of non-coding RNAs and RNA modifiers in cancer therapy resistance[J]. Mol Cancer, 2020, 19(1): 47. doi: 10.1186/s12943-020-01171-z
[9]	XIE Y, DANG W, ZHANG S W, et al. The role of exosomal noncoding RNAs in cancer[J]. Mol Cancer, 2019, 18(1): 37. doi: 10.1186/s12943-019-0984-4
[10]	MAO Q X, LV M H, LI L F, et al. Long intergenic noncoding RNA 00641 inhibits breast cancer cell proliferation, migration, and invasion by sponging miR-194-5p[J]. J Cell Physiol, 2020, 235(3): 2668-2675. doi: 10.1002/jcp.29170
[11]	CHEN C, WANG X, LIU T Z, et al. Overexpression of long non-coding RNA RP11-363E7.4 inhibits proliferation and invasion in gastric cancer[J]. Cell Biochem Funct, 2020, 38(7): 921-931. doi: 10.1002/cbf.3514
[12]	IACCARINO I, KLAPPER W. LncRNA as cancer biomarkers[J]. Methods Mol Biol, 2021, 2348: 27-41.
[13]	吴昊明, 傅广波. 长链非编码RNA SChLAP1表达水平在前列腺癌组织中的临床意义及预后关系[J]. 实用临床医药杂志, 2022, 26(5): 30-33, 38. doi: 10.7619/jcmp.20214427
[14]	WANG Y, LI X, CHEN W, et al. The common region of lncRNAs UCA1 and UCA1α contributes to the bladder cancer tumorigenesis[J]. Eur J Cancer Prev, 2021, 30(5): 389-392. doi: 10.1097/CEJ.0000000000000642
[15]	QUAN J, PAN X, ZHAO L W, et al. LncRNA as a diagnostic and prognostic biomarker in bladder cancer: a systematic review and meta-analysis[J]. Onco Targets Ther, 2018, 11: 6415-6424. doi: 10.2147/OTT.S167853
[16]	DING Z S, YING W W, HE Y H, et al. lncRNA-UCA1 in the diagnosis of bladder cancer: a meta-analysis[J]. Medicine, 2021, 100(11): e24805. doi: 10.1097/MD.0000000000024805
[17]	ALIPOOR B, PARVAR S N, SABATI Z, et al. An updated review of the H19 lncRNA in human cancer: molecular mechanism and diagnostic and therapeutic importance[J]. Mol Biol Rep, 2020, 47(8): 6357-6374. doi: 10.1007/s11033-020-05695-x
[18]	GHAFOURI-FARD S, ESMAEILI M, TAHERI M. H19 lncRNA: roles in tumorigenesis[J]. Biomed Pharmacother, 2020, 123: 109774. doi: 10.1016/j.biopha.2019.109774
[19]	PAN J F, HUANG Z D, LIN H C, et al. M7G-Related lncRNAs predict prognosis and regulate the immune microenvironment in lung squamous cell carcinoma[J]. BMC Cancer, 2022, 22(1): 1132. doi: 10.1186/s12885-022-10232-z
[20]	CHEN Y, WANG D, SHU T, et al. Circular RNA_0000326 promotes bladder cancer progression via microRNA-338-3p/ETS Proto-Oncogene 1/phosphoinositide-3 kinase/Akt pathway[J]. Bioengineered, 2021, 12(2): 11410-11422. doi: 10.1080/21655979.2021.2008738

施引文献(7)

期刊类型引用(6)

1.	黄晨岚. 25羟维生素D、超敏c反应蛋白、估计肾小球滤过率与脑萎缩相关性的临床研究. 黑龙江医药. 2024(01): 44-47 . 百度学术
2.	桂千，侯晓夏，徐勤荣，冯红选，吴冠会，卢艳丽，程庆璋. 脑微出血与非关键部位脑梗死患者认知功能的相关性. 实用临床医药杂志. 2023(13): 43-47+52 . 本站查看
3.	蹇秋枫，徐荣华，姚倩，周媛媛. 中国老年脑卒中患者认知障碍患病率和影响因素的Meta分析. 中国全科医学. 2023(32): 4070-4079+4088 . 百度学术
4.	李梦洁，肖佳男，刘文娟，练芮含，张丽秀. 外周血1, 25-(OH)_2D_3、神经元特异性烯醇化酶、神经胶质纤维酸性蛋白在小儿复杂性惊厥中的表达意义. 中国儿童保健杂志. 2023(11): 1254-1259 . 百度学术
5.	张丽. 脑梗死后认知功能损害与神经功能恢复及生活质量的相关性研究. 临床研究. 2022(08): 77-80 . 百度学术
6.	雷霄明，薛孟周，夏彬，李晶，郭平平. 血清25-羟维生素D和脂联素与脑白质损伤患者认知功能的相关性研究. 实用临床医药杂志. 2022(19): 33-37 . 本站查看