淋巴细胞亚群及免疫球蛋白在孤独症谱系障碍儿童中的表达及临床意义

郭雪松; 缪文静; 冯秀娟; 王大海; 王春艳; 陈丽晔

doi:10.7619/jcmp.20233562

淋巴细胞亚群及免疫球蛋白在孤独症谱系障碍儿童中的表达及临床意义

The expression and clinical significance of lymphocyte subsets and immunoglobulins in children with autism spectrum disorder

摘要

摘要:
目的探讨淋巴细胞亚群绝对计数及免疫球蛋白(Ig)在孤独症谱系障碍(ASD)儿童外周血中的表达水平及临床意义。
方法选取75例ASD患儿纳入研究组，另选取75例健康体检儿童纳入对照组，采用流式细胞技术检测2组儿童外周血中淋巴细胞亚群(CD3⁺、CD4⁺、CD8⁺、CD19⁺、CD56⁺CD16⁺)绝对计数及IgA、IgG、IgM水平, 采用Pearson相关性分析探讨各检测指标与儿童孤独症评定量表(CARS)总分的相关性。
结果研究组CD3⁺、CD4⁺绝对计数依次为(62.49±8.71)、(34.87±7.86)个/μL, 分别低于对照组的(67.73±5.11)、(39.56±4.60)个/μL, 差异有统计学意义(P < 0.05); 2组CD8⁺、CD19⁺、CD56⁺CD16⁺绝对计数比较, 差异无统计学意义(P>0.05)。研究组IgA、IgG、IgM水平依次为(0.79±0.33)、(8.39±2.03)、(0.95±0.27) g/L, 分别低于对照组的(0.94±0.29)、(9.28±1.37)、(1.16±0.39) g/L, 差异有统计学意义(P < 0.05)。Pearson相关性分析结果显示, IgA水平与CARS总分呈显著负相关(r=-0.317, P=0.034), CD3⁺、CD4⁺、CD8⁺、CD19⁺、CD56⁺CD16⁺绝对计数和IgG、IgM水平均与CARS总分无相关性(P>0.05)。
结论淋巴细胞亚群及Ig表达水平异常所致免疫功能障碍与儿童ASD的发生密切相关, CD3⁺、CD4⁺、IgA、IgG和IgM可作为筛查儿童ASD免疫学病因的潜在生物学指标, 且IgA可作为有免疫学病因的ASD患儿的疗效判断指标。

Abstract:
Objective To investigate the expression levels and clinical significance of absolute counts of lymphocyte subsets and immunoglobulins (Ig) in peripheral blood of children with autism spectrum disorder (ASD).
Methods Seventy-five children with ASD were selected as study group, and another 75 healthy children who underwent physical examination were selected as control group. Flow cytometry was used to detect the absolute counts of lymphocyte subsets (CD3⁺, CD4⁺, CD8⁺, CD19⁺, CD56⁺CD16⁺) and IgA, IgG, IgM levels in peripheral blood of both groups of children. Pearson correlation analysis was used to explore the correlation between each detection index and the total score of the Childhood Autism Rating Scale (CARS).
Results The number of absolute counts of CD3⁺ and CD4⁺ in the study group were (62.49±8.71) and (34.87±7.86) in one μL, respectively, which were separately lower than (67.73±5.11) and (39.56±4.60)in one μL in the control group (P < 0.05). There were no significant differences in the absolute counts of CD8⁺, CD19⁺, CD56⁺CD16⁺ between the two groups (P>0.05). The levels of IgA, IgG, IgM in the study group were (0.79±0.33), (8.39±2.03), (0.95±0.27), respectively, which were lower than (0.94±0.29), (9.28±1.37), (1.16±0.39) in the control group (P < 0.05). Pearson correlation analysis showed that IgA level was significantly negatively correlated with CARS total score (r= -0.317, P=0.034), while the absolute counts of CD3⁺, CD4⁺, CD8⁺, CD19⁺, CD56⁺CD16⁺ and IgG, IgM levels had no correlation with CARS total score (P>0.05).
Conclusion Abnormal expression levels of lymphocyte subsets and Ig-mediated immune dysfunction are closely related to the occurrence of ASD in children. CD3⁺, CD4⁺, IgA, IgG, and IgM can be used as potential biological indicators for screening the immunological etiology of ASD in children, and IgA can be used as an indicator for evaluating the efficacy of ASD children with immunological etiology.

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