Abstract: We aimed at the methed for prenatal diagnosis of Wilan disease. Three Amp - FLP polymorphic sites on chromosome 13 were used as genetic markers. Haplotype segregation analysis were carried out in prenatal diagnosis of three WD families. It is concluded that 2 feus are carriers, and 1 fetus is WD patient. The result suggests that using these intragenis markers allows rapid, easy and accurate analysis and prenatal diagnosis in WD families .