应用聚合酶链反应检测南京地区TT病毒感染

TT VIRUS INFECTION IN NANJING AREA DETECTED BY POLYAERASE CHAIN REACTION

摘要: 目的：了解南京地区TT病毒感染情况。方法：采用巢式PCR方法检测血清标本中TTV－DNA。结果：163例病毒性肝炎患者血清标本中，TTV－DNA总检出率为21.5％（35／163），其中甲型肝炎13.3％（4／30），乙型肝炎21.3％（16／75），丙型肝炎20.0％（3／15），戊型肝炎5.3％（1／19），非甲－庚型肝炎45.8％（11／24）。结论：南京地区存在TTV感染，TTV是导致非甲－庚型肝炎的重要病因，TTV可能存在非血源性传播途径。

Abstract: Aim: To investigate the infection of TT virus (TTV) in Nanjing, Jiangsu Province,China. Methods: To apply nested PCR in the detection of TTV DNA in serum sam - ples. Results: of163 cases which have been diagnosed as viral Hepatitis, the total detection rate of TTV DNA was 21. 5%(35/163), of which the detection rates were 13. 3% (4/30) in hepatitis A, 21. 3% (16/75) in hepati tis B, 20. 0% (3/15) in hepatitis C, 5. 3% (1/19) in hepatitis E and 45. 8% (11/24) in nonA to G hepatitis, respectively. Conclusion: TTV infection has been found in Nanging area. TTV maybe the key cause of nonA to G hepa - titis and may have non transfusion transmitted pathway.