Abstract: Objective To observe multi-department combined thermal preservation on serum inflammatory factors in patients undergoing laparoscopic colorectal cancer surgery. Methods A total of 90 patients undergoing laparoscopic colorectal cancer surgery in Subei People's Hospital were selected as subjects, and were randomly divided into A group(conventional thermal preservation), B group(intraoperative thermal preservation)and C group(multi-department combined thermal preservation). Blood samples of three groups were collected, and the levels of serum interleukin-6(IL-6), C reactive protein(CRP), tumor necrosis factor-α(TNF-α)in each group were detected on the first day before operation(T₀), and the first day(T₁), the third day(T₂)and the fifth day(T₃)after operation. Results At T₁, T₂ and T₃, the levels of IL-6, CRP and TNF-α in the C group were significantly lower than those in the A and B groups(P<0.05). Compared with T₀, IL-6, CRP and TNF-α levels in the three groups were increased at different time points after operation, except for the level of TNF-α at T₃ in group C(P<0.05). At T₁, T₂ and T₃, the levels of IL-6, CRP and TNF-α in group C were significantly lower than those in group A and B(P<0.05). Conclusion Combined heat preservation measures of multiple departments can reduce the occurrence of hypothermia and - relieve immune stress damage.