Abstract: Objective To investigate the value of dynamic monitoring of 1, 3-β-D-glucan(BDG)in evaluating the efficiency of drugs for treatment of premature infants with fungal septicemia. Methods Plasma levels of BDG of 30 premature infants with fungal septicemia in Neonatal Intensive Care Unit(NICU)of Hunan Provincial Children's Hospital from January 2018 to December 2019 were dynamically monitored. Results After antifungal treatment, plasma BDG concentration in 28 cases with effective therapeutic result decreased significantly, among which 8 cases decreased to normal level; 2 dead cases showed progressive increase in plasma BDG, and were in a high level. The duration of antibiotic treatment in children with BDG not decreasing to normal level was significantly longer than that in children with BDG decreasing to normal level(P<0.05). Conclusion Dynamic monitoring of plasma BDG level can evaluate the efficacy of selected antifungal drugs, and also has a certain value in evaluating the prognosis of children with fungal septicemia.