高级检索
徐珍, 张玉芹, 韩书清. 乙醇和锌离子对爪蟾卵母细胞表达的5'-三磷酸腺苷门控P2X3受体的影响[J]. 实用临床医药杂志, 2021, 25(21): 15-20. DOI: 10.7619/jcmp.20212695
引用本文: 徐珍, 张玉芹, 韩书清. 乙醇和锌离子对爪蟾卵母细胞表达的5'-三磷酸腺苷门控P2X3受体的影响[J]. 实用临床医药杂志, 2021, 25(21): 15-20. DOI: 10.7619/jcmp.20212695
shu
XU Zhen, ZHANG Yuqin, HAN Shuqing. Effects of ethanol and zinc ions on 5'- adenosine triphosphate gated P2X3 receptor expressed in Xenopus oocytes[J]. Journal of Clinical Medicine in Practice, 2021, 25(21): 15-20. DOI: 10.7619/jcmp.20212695
Citation: XU Zhen, ZHANG Yuqin, HAN Shuqing. Effects of ethanol and zinc ions on 5'- adenosine triphosphate gated P2X3 receptor expressed in Xenopus oocytes[J]. Journal of Clinical Medicine in Practice, 2021, 25(21): 15-20. DOI: 10.7619/jcmp.20212695
shu

乙醇和锌离子对爪蟾卵母细胞表达的5'-三磷酸腺苷门控P2X3受体的影响

Effects of ethanol and zinc ions on 5'- adenosine triphosphate gated P2X3 receptor expressed in Xenopus oocytes

    摘要
  • 摘要:
      目的  探讨乙醇和锌离子（Zn2+）对爪蟾卵母细胞表达的重组P2X3受体的影响。
      方法  使用双极电压钳技术检测乙醇和Zn2+对爪蟾卵母细胞表达的P2X3受体介导的5'-三磷酸腺苷（ATP）门控电流的影响。实验共分3组，第1组研究不同浓度乙醇对爪蟾卵母细胞表达的P2X3受体介导的ATP电流的影响，存在或不存在乙醇的情况下，P2X3受体的ATP量-效曲线；第2组研究不同浓度Zn2+对爪蟾卵母细胞表达的P2X3受体介导的ATP电流的影响，存在或不存在Zn2+的情况下，P2X3受体的ATP量-效曲线；第3组研究乙醇和Zn2+共同作用于爪蟾卵母细胞表达的ATP门控P2X3受体的影响。
      结果  乙醇（5~200 mmol/L）可逆性地增强了爪蟾卵母细胞中表达的P2X3受体的ATP门控功能，乙醇以变构的方式增强ATP效应，乙醇不会改变Hill系数或ATP最大效应（Emax）。Zn2+（1~300 μmol/L）可逆性地增强了爪蟾卵母细胞中表达的P2X3受体的ATP门控功能，乙醇和Zn2+联合应用导致协同作用。乙醇增强了Zn2+对P2X3受体介导的ATP门控电流的最大效应，这表明乙醇和Zn2+作用于不同的位点。
      结论  乙醇和Zn2+可能作用于P2X3受体上的不同位点，但乙醇在P2X受体上起作用的机制和位点尚未明确，后续研究可以使用嵌合方法和定点突变来识别和分析P2X受体中乙醇潜在的作用位点。

     

    Abstract:
      Objective  To investigate the effects of ethanol and zinc ions (Zn2+) on the recombinant P2X3 receptor expressed in Xenopus oocytes.
      Methods  Bipolar voltage clamp technique was used to detect the effects of ethanol and Zn2+ on P2X3 receptor mediated 5'- adenosine triphosphate (ATP) gated current expressed in Xenopus oocytes. The experiment was divided into three groups. The first group explored the effects of different concentrations of ethanol on the expression of P2X3 receptor mediated ATP current in Xenopus oocytes, and the ATP concentration-response curve of P2X3 receptor in the presence and absence of ethanol. In the second group, the effects of different concentrations of Zn2+ on the expression of P2X3 receptor mediated ATP currents in Xenopus oocytes and the ATP concentration-response curve of P2X3 receptor in the presence and absence of Zn2+ were investigated. The third group studied the effect of ethanol and Zn2+ on ATP gated P2X3 receptor expressed in Xenopus oocytes.
      Results  Ethanol (5~200 mmol/L) reversibly enhanced the ATP-gated function of P2X3 receptor expressed in Xenopus oocytes, ethanol increased the ATP effect in an allosteric manner, and ethanol did not change the Hill coefficient or the maximum ATP effect (Emax). Zn2+ (1~300 μmol/L) reversibly enhanced the ATP-gated function of P2X3 receptor expressed in Xenopus oocytes, the combination of ethanol and Zn2+ led to a synergistic effect. Ethanol increased the maximum effect of Zn2+ on P2X3 receptor mediated ATP-gated current, which suggested that ethanol and Zn2+acted on different sites.
      Conclusion  This study reveals that ethanol and Zn2+ may act on different sites of P2X3 receptor, but the mechanism and site of ethanol acting on P2X receptor are not clear. In subsequent studies, chimeric method and site directed mutation can be used to identify and analyze the potential action sites of ethanol in P2X receptor.

     

    • HTML全文
    • 参考文献(19)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回