双黄连雾化吸入对小鼠H9N2禽流感病毒感染的疗效研究

丘国清; 杜秋伶; 江海明; 黄文博; 杨子峰

doi:10.7619/jcmp.20212844

双黄连雾化吸入对小鼠H9N2禽流感病毒感染的疗效研究

Efficacy of Shuanghuanglian aerosol inhalation in treatment of mice with H9N2 avian influenza virus infection

摘要

摘要:
目的探讨双黄连雾化吸入（SHL Inh）对小鼠H9N2亚型禽流感病毒感染的疗效。
方法将45只BALB/c小鼠随机分为正常对照组、病毒对照组、奥司他韦组（阳性药对照）、双黄连腹腔注射（SHL Ip）组和SHL Inh组，每组9只。正常对照组经鼻滴入磷酸盐缓冲液（PBS），其余4组均经鼻接种等量H9N2禽流感病毒。奥司他韦组、SHL Ip组和SHL Inh组在接种病毒后分别给予相应药物治疗，正常对照组和病毒对照组给予等量蒸馏水，连续给药5 d。每日记录小鼠体质量，接种后第5天处死，取肺称重，取部分肺组织制作HE病理切片，其余肺组织匀浆后测定病毒滴度、炎症因子表达水平。
结果病毒对照组和SHL Ip组小鼠肺指数均高于正常对照组，差异有统计学意义（P < 0.01）。SHL Inh组肺指数低于病毒对照组，高于SHL Ip组，差异有统计学意义（P < 0.01）。病毒对照组肺病毒滴度为（4.06±0.41）lgTCID₅₀/g，奥司他韦组、SHL Inh组和SHL Ip组病毒滴度依次为（3.29±0.27）、（3.44±0.46）、（3.33±0.43）lgTCID₅₀/g，均低于病毒对照组病毒滴度，差异有统计学意义（P < 0.05或P < 0.01）。SHL Inh并未改善小鼠体质量下降，也未降低肺炎症因子水平。SHL Ip组与SHL Inh组在体质量下降、肺炎症因子水平、肺病毒滴度和肺病理炎症方面的差异无统计学意义（P>0.05）。
结论 SHL Inh具有抗H9N2亚型禽流感病毒的疗效，且雾化给药与注射给药在降低病毒滴度方面的疗效相当。

Abstract:
Objective To explore the efficacy of Shuanghuanglian aerosol inhalation (SHL Inh) in treatment of mice with H9N2 subtype avian influenza virus infection.
Methods Forty-five BALB/c mice were randomly divided into normal control group, virus control group, oseltamivir group (controls with positive drugs), SHL intraperitoneal injection (SHL Ip) group and SHL Inh group, with 9 mice in each group. The normal control group was injected with phosphate buffer (PBS) through the nose, and the other four groups were inoculated with the same amount of H9N2 avian influenza virus through the nose. Oseltamivir group, SHL Ip group and SHL Inh group were treated with corresponding drugs respectively after inoculation with virus, the normal control group and virus control group were given the same amount of distilled water for 5 days. The body weights of mice were recorded every day, and the mice were killed on the 5^th day after inoculation. The lungs were weighed, and partial lung tissues were taken to make HE pathological sections, and the virus titer and the expression level of inflammatory factors were measured after homogenization of other lung tissues.
Results The lung indexes of mice in the virus control group and the SHL Ip group were significantly higher than that in the normal control group (P < 0.01). The lung index in the SHL Inh group was significantly lower than that in the virus control group and higher than that in the SHL Ip group (P < 0.01). The pulmonary virus titer in the virus control group was (4.06±0.41) lgTCID₅₀/g, while the virus titers in the oseltamivir group, SHL Inh group and SHL Ip group were (3.29±0.27), (3.44±0.46) and (3.33±0.43) lgTCID₅₀/g, which were significantly lower than that in the virus control group (P < 0.05 or P < 0.01). SHL Inh did not improve the decline of body mass or reduce the level of pneumonia factors in mice. There were no significant differences in body mass decline, pneumonia factors levels, pulmonary virus titer and pulmonary pathological inflammation between the SHL Ip group and the SHL Inh group (P>0.05).
Conclusion SHL Inh has the effect of anti-H9N2 subtype avian influenza virus, and the effect of atomization administration is equivalent to that of injection administration in reducing virus titer.

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