Objective  To investigate the effect of long intergenic non-coding RNA LINC01139 (LINC01139) in regulating the proliferation, migration and invasion of oral squamous cell carcinoma cisplatin(DDP)-resistant cells(CAL-27/DDP) by regulating microRNA-300 (miR-300).

  Methods  Real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression levels of LINC01139 and miR-300 in CAL-27/DDP and its parental cell line CAL-27. CAL-27/DDP was divided into DDP+si-NC, DDP+si-LINC01139, DDP+miR-NC, DDP+miR-300, DDP+ si-LINC01139 +anti-miR-NC, DDP+si-LINC01139+anti-miR-300 groups. The methyl thiazolyl tetrazolium (MTT) method was used to detect the cell proliferation inhibition rate; Transwell assay was used to detect the number of migrating and invading cells. The dual luciferase reporting experiment and qRT-PCR were used to detect the targeting relationship between LINC01139 and miR-300.

  Results  Compared with CAL-27 cells, the expression of LINC01139 in CAL-27/DDP was increased, while the expression of miR-300 was decreased (P < 0.05). Compared with the DDP+si-NC group, the proliferation inhibition rate of CAL-27/DDP in the DDP+si-LINC01139 group was increased, and the migrating and invading cells were reduced (P < 0.05). Compared with the DDP+miR-NC group, the proliferation inhibition rate of CAL-27/DDP in the DDP+miR-300 group was increased, and the number of migrating and invading cells was reduced (P < 0.05). Compared with the DDP+ si-LINC01139+anti-miR-NC group, the proliferation inhibition rate of CAL-27/DDP in the DDP+si-LINC01139+anti-miR-300 group was decreased, the migrating and invading cells were increased (P < 0.05).

  Conclusion  Inhibition of LINC01139 inhibits the proliferation, migration and invasion of oral squamous cell carcinoma cisplatin-resistant cells by up-regulating miR-300.