可注射藻蓝蛋白纳米复合水凝胶的制备及其对伤口愈合效果研究

龙金华; 廖影; 滕丽晶; 曾柱

doi:10.7619/jcmp.20232767

可注射藻蓝蛋白纳米复合水凝胶的制备及其对伤口愈合效果研究

Research of preparation of injectable phycocyanin nanocomposite hydrogels and its application effect in wound healing

摘要

摘要:
目的探讨基于纳米黏土氧化的聚多巴胺（LP）和藻蓝蛋白（CPC），通过超分子相互作用制备剪切变稀CPC/LP纳米复合水凝胶用于伤口愈合的效果。
方法采用纳米黏土分散液弱碱性含氧环境预聚合多巴胺单体形成LP聚集体，并将其与多功能CPC混合，通过静电作用、席夫碱等形成剪切变稀纳米复合水凝胶，并系统研究水凝胶的微结构、流变性能、血液相容性以及促伤口愈合效果。
结果 CPC/LP纳米复合水凝胶的凝胶化性能与LP的含量有关，当LP含量为3%，CPC含量为0.5%时，能够形成稳定的凝胶；流变测试结果表明纳米复合水凝胶具有剪切变稀和自修复的性能；血液相容性实验结果表明，纳米复合水凝胶溶血率低于5%，具有良好的血液相容性；此外，纳米复合水凝胶能够通过较小的针头注射到损伤部位，促进创面愈合。
结论 CPC/LP纳米复合水凝胶呈现优异的可注射性能，可有效促进伤口愈合。

Abstract:
Objective To investigate the effect of preparation of shear-thinning CPC/LP nanocomposite hydrogel for wound healing by supramolecular interaction of polydopamine (LP) and phycocyanin (CPC) based on nano-clayoxidation.
Methods LP aggregates were formed by polymerization of dopamine monomers in weak alkaline oxygen-containing environment of nano-clay dispersion, and then mixed it with multifunctional CPC to form shear-thinning nanocomposite hydrogels by electrostatic action and Schiff base. The microstructure, rheological properties, blood compatibility and wound healing effect of hydrogel were studied systematically.
Results The gelation performance of CPC/LP nanocomposite hydrogel was related to LP content. When LP content was 3% and CPC content was 0.5%, stable gel could be formed; rheological test results showed that the nanocomposite hydrogel had the properties of shear thinning and self-healing; the results of blood compatibility test showed that the hemolysis rate of the nanocomposite hydrogel was less than 5%, and it had good blood compatibility. In addition, the nanocomposite hydrogel could be injected into the injury site through a smaller needle to promote wound healing.
Conclusion CPC/LP nanocomposite hydrogel exhibits excellent injectable properties and can effectively promote wound healing.

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