微小RNA-451在小细胞性贫血及慢性感染贫血儿童血清中的表达及临床意义

徐金梅; 张利兵; 郁多男

doi:10.7619/jcmp.20233257

微小RNA-451在小细胞性贫血及慢性感染贫血儿童血清中的表达及临床意义

Expression and clinical significance of microRNA-451 in children with microcytic anemia and chronic infectious anemia

摘要

摘要:
目的分析微小RNA-451(miR-451)在小细胞性贫血及慢性感染贫血儿童血清中的表达情况, 探讨miR-451与红细胞相关参数的相关性及其对儿童贫血的诊断价值。
方法采用实时荧光定量聚合酶链反应(qRT-PCR)分别检测小细胞性贫血组(n=80)、慢性感染贫血组(n=80)、健康对照组(n=80)儿童血清miR-451表达水平; 采用Pearson相关分析法探讨血清miR-451与红细胞相关参数红细胞分布宽度(RDW)、红细胞(RBC)、平均红细胞血红蛋白浓度(MCHC)、血红蛋白(Hb)、平均红细胞血红蛋白含量(MCH)、平均红细胞体积(MCV)的相关性; 绘制受试者工作特征(ROC)曲线，分析miR-451对小细胞性贫血、慢性感染贫血、贫血严重程度的诊断价值; 采用多因素Logistic回归分析探讨儿童发生小细胞性贫血、慢性感染贫血的影响因素。
结果小细胞性贫血组、慢性感染贫血组儿童血清miR-451表达水平低于健康对照组，差异有统计学意义(P < 0.05); miR-451与RDW呈负相关(r=-0.388, P < 0.05), 与Hb、RBC、MCHC、MCH、MCV分别呈正相关(r=0.402、0.393、0.391、0.360、0.323, P < 0.05)。ROC曲线分析结果显示, 血清miR-451诊断小细胞性贫血、慢性感染贫血的曲线下面积分别为0.718、0.735, 诊断价值高于红细胞相关参数RDW、Hb、RBC、MCHC、MCH、MCV, 差异有统计学意义(P < 0.05)。多因素Logistic回归分析结果显示，体质量指数、RDW、Hb、RBC、MCHC、MCH、MCV、miR-451均为儿童发生小细胞性贫血、慢性感染贫血的影响因素(P < 0.05)。随着儿童贫血程度的加重，血清miR-451表达水平逐渐降低，差异有统计学意义(P < 0.05); ROC曲线分析结果显示，血清miR-451水平对儿童贫血严重程度具有较高的诊断价值。
结论 miR-451在小细胞性贫血、慢性感染贫血儿童血清中表达降低，且其表达水平与红细胞相关参数水平存在相关性，血清miR-451对小细胞性贫血、慢性感染贫血和贫血严重程度均具有较高的诊断价值。

Abstract:
Objective To analyze the expression of microRNA-451 (miR-451) in children with microcytic anemia and chronic infectious anemia, and to explore the correlation between miR-451 and erythrocyte-related parameters and its diagnostic value for anemia in children.
Methods The expression levels of miR-451 in serum were detected in children with microcytic anemia (n=80), chronic infectious anemia (n=80), and healthy controls (n=80) using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The correlation between serum miR-451 and erythrocyte-related parametersred blood cell distribution width (RDW), red blood cells (RBC), mean corpuscular hemoglobin concentration (MCHC), hemoglobin (Hb), mean corpuscular hemoglobin content (MCH), mean corpuscular volume (MCV)was analyzed using Pearson correlation analysis. The receiver operating characteristic (ROC) curve was plotted to analyze the diagnostic value of miR-451 for microcytic anemia, chronic infectious anemia, and the severity of anemia. Multivariate Logistic regression analysis was used to investigate the influencing factors for the occurrence of microcytic anemia and chronic infectious anemia in children.
Results The expression levels of serum miR-451 in the microcytic anemia group and chronic infectious anemia group were lower than those in the healthy control group(P < 0.05). The miR-451 was negatively correlated with RDW (r=-0.388, P < 0.05), and positively correlated with Hb, RBC, MCHC, MCH, and MCV (r=0.402, 0.393, 0.391, 0.360, 0.323, P < 0.05). ROC curve analysis showed that the areas under the curve of serum miR-451 for diagnosing microcytic anemia and chronic infectious anemia was 0.718 and 0.735, respectively, which was higher than that of erythrocyte-related parameters such as RDW, Hb, RBC, MCHC, MCH, and MCV (P < 0.05). Multivariate Logistic regression analysis showed that body mass index, RDW, Hb, RBC, MCHC, MCH, MCV, and miR-451 were influencing factors for the occurrence of microcytic anemia and chronic infectious anemia in children (P < 0.05). With the aggravation of anemia in children, the expression level of serum miR-451 gradually decreased (P < 0.05). ROC curve analysis showed that the level of serum miR-451 had a high diagnostic value for the severity of anemia in children.
Conclusion The expression of miR-451 is reduced in serum of children with microcytic anemia and chronic infectious anemia, and its expression level is correlated with erythrocyte-related parameter levels. Serum miR-451 has a high diagnostic value for microcytic anemia, chronic infectious anemia, and the severity of anemia.

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