Toll样受体4/核因子-κB p65通路对轮状病毒肠炎患儿肝损害的影响

申永静; 杜宝静; 商敬敏; 韩冰; 苗院敏

doi:10.7619/jcmp.20240098

Toll样受体4/核因子-κB p65通路对轮状病毒肠炎患儿肝损害的影响

Effect of toll-like receptor 4/nuclear factor κB p65 pathway on liver damage in children with rotavirus enteritis

摘要

摘要:
目的探讨轮状病毒（RV）肠炎患儿Toll样受体4（TLR4）/核因子-κB（NF-κB）p56通路对肝损害的影响。
方法选取50例RV肠炎患儿为RV组，另选取同期50例RV感染阴性的肠炎患儿为对照组。采用全自动生化分析仪检测患儿肝功能指标谷草转氨酶（AST）和谷丙转氨酶（ALT）水平。采用聚合酶链式反应（PCR）检测外周血单核细胞（PMBC）的TLR4 mRNA、NF-κB mRNA表达；采用蛋白质印迹法检测NF-κB p65蛋白表达水平。比较2组患儿血清AST、ALT水平和肝损害发生率。比较2组患儿PMBC的TLR4 mRNA、NF-κB mRNA和NF-κB p65蛋白表达。比较RV组中伴肝损害患儿和无肝损害患儿PMBC的TLR4 mRNA、NF-κB mRNA和NF-κB p65蛋白表达水平。采用Pearson相关分析法分析RV肠炎患儿肝功能与TLR4/NF-κB p65通路的相关性。
结果 RV组患儿血清AST、ALT水平及肝损伤发生率高于对照组，差异有统计学意义（P < 0.05）。RV组患儿PBMC的TLR4 mRNA、NF-κB mRNA和NF-κB p65蛋白表达高于对照组，差异有统计学意义（P < 0.05）。RV组中，伴肝损害患儿PBMC的TLR4 mRNA、NF-κB mRNA和NF-κB p65蛋白表达高于无肝损害患儿，差异有统计学意义（P < 0.05）。RV肠炎患儿血清AST、ALT与TLR4 mRNA、NF-κB mRNA和NF-κB p65蛋白表达呈正相关（P < 0.05）。
结论 RV肠炎患儿肝损害发生率较高，且与TLR4/NF-κB p65通路相关。TLR4/NF-κB p65通路过度激活可能是RV肠炎患儿发生肝损害的原因之一。

Abstract:
Objective To investigate effect of toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) p56 pathway on liver damage in children with rotavirus (RV) enteritis.
Methods A total of fifty children with RV enteritis were selected as RV group, while another 50 children with RV negative enteritis during the same period were selected as control group. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured by automatic biochemical analyzer. TLR4 mRNA and NF-κB mRNA expression in peripheral blood mononuclear cells (PMBC) were detected by polymerase chain reaction (PCR); the expression level of NF-κB p65 protein was detected by Western Blot. The levels of serum AST, ALT and the incidence of liver damage were compared between the two groups. TLR4 mRNA, NF-κB mRNA and NF-κB p65 protein expression in PMBC were compared between the two groups. The expression levels of TLR4 mRNA, NF-κB mRNA and NF-κB p65 protein in PMBC were compared between children with and without liver damage in RV group. The correlation between liver function and TLR4/NF-κB p65 pathway in children with RV enteritis was analyzed by Pearson correlation method.
Results The levels of serum AST and ALT as well as the incidence of liver injury in the RV group were significantly higher than those in the control group (P < 0.05). The expressions of TLR4 mRNA, NF-κB mRNA and NF-κB p65 protein of PBMC in the RV group were significantly higher than those in the control group (P < 0.05). In the RV group, the expressions of TLR4 mRNA, NF-κB mRNA and NF-κB p65 protein of PBMC in children with liver damage were significantly higher than those in children without liver damage (P < 0.05). Serum AST and ALT were positively correlated with TLR4 mRNA, NF-κB mRNA and NF-κB p65 protein expression in children with RV enteritis (P < 0.05).
Conclusion The incidence of liver damage is higher in children with RV enteritis, which is related to TLR4/NF-κB p65 pathway. Overactivation of TLR4/NF-κB p65 pathway may be one of the causes of liver damage in children with RV enteritis.

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