GM-CSF基因转染瘤苗对小鼠T淋巴细胞亚群及生存期的影响

THE EXPERIMENTAL STUDY ON THE CHANGE OF T-LYMPHOCYTE SUBSETS OF MICE AFTER TREATMENT WITH GM-CSF-TRANSFECTED TUMOR CELLS

摘要: 将携带有人粒细胞-巨噬细胞集落刺激因子（GM—CSF）基因的重组腺病毒载体转集BAI．B／c小鼠肝癌细胞株（H22），体外经60Coγ射线灭活后制成瘤苗，用以治疗荷瘤小鼠。应用流式细胞仪（FCM）测定荷瘤小鼠外周血T淋巴细胞亚群的变化，并观察荷瘤小鼠生存期。结果发现，GM－CSF基因转染瘤苗能显著提高小鼠外周血CDs8＋T淋巴细胞亚群的含量，荷瘤小鼠生存期显著延长。结果表明：GM—CSF基因转染瘤苗能诱导出有效的抗肿瘤免疫反应，提示应用该疗法进行肿瘤基因治疗的可行性。

Abstract: H22 cells, a hepatocellular carcinoma cell line of BALB/c mice originally, were transfected with human GM - CSF via recombinant adenoviral and then irradiated byo'Coy ray. Bearing mice were treated by above tumor vaccine. We determined the lymphocytes subsets in peripheral blood of bearingmice by using Flow Cytometer (FCM). We also observed the survival stage of ab0ve animals. Our resultsfollowed that treatment with GM - CSF - trans fected, irradiated H22 cells not only increased the percentageof CD + T - lymphocytes in peripheral blood, but also significantly prolonged survivals of tumor bearingmice. The result suggests that irradiated, GM - CSF - trans fected tumor cells could induce effective antitumor immunity, which indicates the feasibility of cancer gene therapy.