GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level

ZHANG Rong; YANG Xinquan; XIONG Ting; TANG Shan; AI Keqing; ZHONG Hongyan; MAO Yongqing; WANG Daxin

doi:10.7619/jcmp.201910015

Journal of Clinical Medicine in Practice > 2019 > 23(10): 53-57. > DOI: 10.7619/jcmp.201910015

ZHANG Rong, YANG Xinquan, XIONG Ting, TANG Shan, AI Keqing, ZHONG Hongyan, MAO Yongqing, WANG Daxin. GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level[J]. Journal of Clinical Medicine in Practice, 2019, 23(10): 53-57. DOI: 10.7619/jcmp.201910015

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GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level

1.
Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001
2.
Xiangya Medical School of Central South University, Changsha, Hunan, 410000
3.
Dalian Medical University, Dalian, Liaoning, 116000

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Received Date: March 15, 2019
Accepted Date: April 16, 2019
Available Online: February 22, 2021
Published Date: May 27, 2019

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Abstract

Abstract

Objective To explore the mechanism of GKT137831 on protecting skeletal muscle cells by atorvastatin intervention.
Methods Atorvastatin was used to intervene C2C12 cells in skeletal muscle for 48 hours. CCK-8 method was used to analyze the effect of atorvastatin on survival rate of skeletal muscle cells. Western blot was used to detect the expression of NADPH Oxidase 4 (Nox4) after atorvastatin intervention. Flow cytometry was used to detect the effect of atorvastatin on glucose uptake and reactive oxygen species (ROS) in skeletal muscle cells. Co-culture of Nox4 inhibitor GKT137831 was used to analyze the effect of atorvastatin on cellular glucose uptake and ROS level.
Results After intervention with atorvastatin on skeletal muscle cells for 48 hours, the glucose uptake ability of C2C12 cells decreased and ROS increased. GKT137831 could alleviate the decrease of glucose uptake and the increase of ROS. CCK-8 results showed that atorvastatin had no effect on cell viability after 48 hours of intervention. Nox4 expression increased after atorvastatin intervention on C2C12 skeletal muscle cells.
Conclusion GKT137831 can alleviate atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level.
- GKT137831,
- atorvastatin,
- reactive oxygen species,
- glucose uptake

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GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level

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