Detection study of gene typing of human papillomavirus of 164 patients with cervical cancer in Gansu province

Objective To study the distribution and the subtypes of human papillomavirus (HPV)infection of female patients with cervical cancer and /or other genital diseases in Gansu province,attempting to provide virological data for the prevention of HPV infection and treatment of cervical cancer.Methods A total of 164 specimens with cervical cancer and 100patients with oth-er genital diseases were detected by flow-through hybridization and gene chip.Results 14 HPV subtypes were detected in the 164 patients with cervical cancer (positive HPV DNA rate:85.4%), in which the 10 HPV subtypes with high risk were HPV-16 (68.3%,112 /164),-52 (6.1%,10 /164),-58,-18,-33 (3.7% each 6 /164),-59 (2.4%,4 /164)and-31,-45 (1.2% each,2 /164) while 4 HPVsubtypesoflowriskwereHPV-6 (1 .2 % ,2 /1 6 4 ),-1 1 (4 .9 % ,8 /1 6 4 ),-4 2 (1.2%,2 /164)and-CP8304 (2.4%,4 /164).9 HPV subtypes were detected in 100 patients with other genital diseases (positive HPV DNA rate:28.0%),in which 8 HPV subtypes with high risk were HPV-16 (12%,12 /100),-52 (6.0%,6 /100),-68 (4.0%,4 /100),-31,-18,-39,-58, and-59 (2.0%each,2 /100)while 1 HPV subtype with low risk was HPV-11 (8.0%,8 /100). Multiple infection rate of cervical cancer was 17.1%(28 /164)and multiple infection rate of genital tractwas6 .0 % (6 /1 0 0 ).Conclusion High risk HPV subtypes infection is the dominant factor for cervical cancer in Gansu province ,in which HPV-1 6 and HPV-5 2 are the most common subtypes.There are certain correlation between HPV infection and other genital diseases.The flow-through hybridization and gene chip technique can be applied to detect HPV of female pa-tients with genital infection to confirm subtype genotypes of HPV and to guide clinic treatment and follow-up visits.