Objective To investigate the possible mechanism of coix seed in inducing apoptosis of gastric cancer cells (SGC-7901).
Methods Different concentrations of coix seed oil (2, 4, 8 mg/mL) were applied to SGC-7901 cells. MTT assay was used to detect the effect of drugs on cell proliferation, and flow cytometry for drug-induced cell apoptosis, scratch test for cell migration inhibited by coix seed oil, Transwell chamber for drug-inhibited cell invasion, and Western blot for expression of related proteins PRMT5, PI3K and AKT.
Results The results of MTT showed that 2, 4 mg/mL of coix seed oil could significantly inhibit the proliferation of SGC-7901 cells, and the inhibition rate of 2 mg/mL was (30.02±1.56)%, which showed significant difference compared to the control group (P < 0.01). The results of flow cytometry showed that the apoptosis rates of coix seed oil cells at concentrations of 2 and 4 mg/mL were (16.25±2.54)%, (12.60±1.12)%, respectively, and which showed a significant difference compared with (2.0±1.22)% in the control group (P < 0.01). The results of cell scratch test showed that the migration of SGC-7901 cells treated with 2, 4 mg/mL of coix seed oil had significant difference when compared to control group (P < 0.01). The results of invasion experiments showed that 2, 4 mg/mL of coix seed oil could significantly inhibit cell invasion, and the number of cell invasion was(134.00±2.86), (167.00±0.99), respectively, which showed significant difference compared to(268.00±2.05)in the control group (P < 0.01). The migration number in 8 mg/mL coix seed oil group was (167±0.99), a significant difference was observed when compared to the control group(P < 0.05). Western blot analysis showed that different concentrations of coix seed oil could significantly down-regulate the expression of PRMT5, PI3K and AKT in SGC-7901 cells.
Conclusion Coix seed oil can significantly inhibit the proliferation, migration and invasion of gastric cancer SGC-7901 cells, its possible mechanism is to down-regulate the signal pathway of PRMT5-PI3K/AKT to inhibit the activation of various anti-apoptotic molecules, induce apoptosis and suppress invasion and metastasis of gastric cancer SGC-7901 cells by down-regulating the PRMT5-PI3K/AKT signaling pathway.
DownLoad: