Objective To establish a Myc-inducible mouse model of B-cell lymphoma.
Methods Mixture of fresh p53-null bone marrow cells and virus-packaging cells producing Myc estrogen receptor (MycER) retrovirus were injected into mice subcutaneously. Tamoxifen (TAM) was injected intra-peritoneally once a day.
Results B lymphomas developed and were growing fast with injection of TAM, while there was no tumor development without injection of TAM. However, tumor growth was dramatically repressed after withdrawal of TAM. Interestingly, tumors grew slowly again three weeks later even without TAM injection, and abnormal acceleration of tumor growth occurred if TAM was re-administrated.
Conclusion In this study, we successfully establish a B-cell lymphoma mouse model with controllable expression of Myc through TAM to regulate the inactivation and activation of Myc.
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