Role of calmodulin-dependent protein kinase Ⅱ in reversingdrug resistance to cisplatin chemotherapy by verapamil in patients with lung adenocarcinoma cells

  Objective  To investigate the role of calmodulin-dependent protein kinase Ⅱ(CaMKⅡ) in reversing drug resistance to cisplatin chemotherapy by verapamil in patients with lung adenocarcinoma cells.

  Methods  In lung adenocarcinoma cell lines (A549), the CCK-8 method was used to detect the ability of verapamil in reversing the drug resistance to cisplatin chemotherapy, the Annexin V-FITC/PI method was used to detect the effect of verapamil on reversing the apoptosis of drug resistance to cisplatin chemotherapy, and the Western blotting was used to detect the expression levels of P-CaMKⅡ/CaMKⅡ protein in reversal process.

  Results  In the A549 cell line, the inhibition rates of DDP group and DDP-VER combination group were (55.00±2.60) % and (32.30±1.80) % respectively, and there was significant difference between the two groups (P < 0.05). The apoptosis rates of DDP group and DDP-VRP combination group were 10.30% and 21.60% respectively, and there was significant difference between the two groups (P < 0.05). Western blotting results showed that the expression level of P-CaMKⅡ/CaMKⅡ protein in A549 cell line in DDP-VRP combination group was obviously lower than that in DDP group.

  Conclusion  In lung adenocarcinoma cells, CaMKⅡ participates in the reversal process of drug resistance to cisplatin chemotherapy by verapamil.