Expression and purification of recombinant human bone morphogenetic protein 10 and it cardiac protection effect

  Objective  To verify the cardiac protection effect of recombinant human bone morphogenetic protein 10 (rhBMP10).

  Methods  The rhBMP10 was expressed and purified by CHO cells, heart failure model was established by repeated administration of doxorubicin, and cardiac function and apoptosis were evaluated by echocardiography and TUNEL staining.

  Results  In this study, rhBMP10 with biological activity was successfully expressed. Cardiac function of doxorubicin-induced mice was severely damaged. Apoptotic rate of cardiomyocytes significantly increased, and cardiac function and cardiomyocytes of rhBMP10-treated mice were not affected significantly.

  Conclusion  The rhBMP10 has a positive effect on reducing heart damage caused by doxorubicin.