Analysis of pathogenic bacteria in 120 patients with urinary tract infection

  Objective  To analyze the distribution characteristics and infection status of pathogenic bacteria in patients with urinary tract infection.

  Methods  A total of 120 patients with urinary tract infection in our hospital were selected. The mid-stream urine samples of the selected patients were collected for identification of pathogens detected by VITEK automatic microbiological analyzer. The minimum concentration of antibacterial agents by broth dilution method, and the drug sensitive test was conducted by K-B disk diffusion method to identify broad spectrum beta-lactamase(ESBLs) strain.

  Results  Pathogenic positive bacteria results were seen in urine samples of 120 patients with urinary tract infection, including 39 strains of Gram-positive bacteria (Enterococcus faecalis and Enterococcus faecalis as major pathogens), 70 strains of Gram-negative bacteria and 11 strains of fungi (Candida albicans as major pathogen), with Gram-negative bacteria accounting for the largest proportion(Escherichia coli and Klebsiella pneumoniae as major pathogen). Thirty-three strains of Escherichia coli producing ESBLs(accounting for 68.75%in obtained strains of Escherichia coli) and seven strains of Klebsiella pneumoniae(accounting for 58.33% in total strains of Klebsiella pneumoniae) producing ESBLs were obtained by drug susceptibility test. The two ESBLs-producing pathogens had high drug resistance to cephalosporins and penicillins, but no resistance to antibiotics such as Penan and meropenem. Drug resistance in ESBLs-producing strains were higher that those of non-ESBLs-producing strains. Enterococcus faecalis and Enterococcus faecalis had no resistance to potent antibiotics such as teicoplanin, vancomycin, less resistance to furantoin and rifampicin and complete resistance to penicillin.

  Conclusion  The main pathogens of urinary tract infections are Gram-negative bacteria, among which Escherichia coli accounts for the most proportion with more strains of ESBLs-producing pathogen and have different degrees of drug resistance to commonly used antibiotics in clinic. Therefore, patients need to carry out pathogenic bacteria identification and drug susceptibility test in the process of diagnosis and treatment of urinary tract infection so as to identify the types of pathogenic bacteria and their drug resistance to select right and reasonable antibiotics for treatment, reduce the abuse of antibiotics, and improve the efficacy of antibiotics.