Research on drug resistance of aneuploid colon cancer cells to cisplatin

Objective To explore drug resistance of aneuploid colon cancer cells to cisplatin. Methods The HCT116 cells with expression of knockdown MAD2 induced by doxycycline(DOX)were designed as DOX(+)group(aneuploid), and HCT116 cells without process of DOX were designed as DOX(-)group(euploid). CCK-8 method was used to process cells in both groups, and CCK-8 test and cytometry were used to analyze the effect of cisplatin on cell growth. Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the expression of pro-apoptotic genes BAX, PUMA and NOXA in the cells. Results Cells were treated with cisplatin at different concentrations. CCK-8 test and cytometry showed that cells in DOX(+)group were more resistant to cisplatin than those in DOX(-)group. Western blot showed that the resistance of cells in DOX(+)group was related to the lower expression of apoptotic protein after cisplatin treatment. The qRT-PCR showed that the expressions of apoptosis gene PUMA, NOXA and BAX in DOX(+)group were significantly lower than those in DOX(-)group(P<0.05 or P<0.01)after process of cisplatin. Conclusion The aneuploid colon cancer cells are resistant to cisplatin treatment.