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ZHANG Hanqing, LIU Ying, FANG Xiao. Research on drug resistance of aneuploid colon cancer cells to cisplatin[J]. Journal of Clinical Medicine in Practice, 2020, 24(1): 42-45. DOI: 10.7619/jcmp.202001011
Citation: ZHANG Hanqing, LIU Ying, FANG Xiao. Research on drug resistance of aneuploid colon cancer cells to cisplatin[J]. Journal of Clinical Medicine in Practice, 2020, 24(1): 42-45. DOI: 10.7619/jcmp.202001011
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Research on drug resistance of aneuploid colon cancer cells to cisplatin

  • 1. Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001;

  • 2. Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001

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  • Received Date: October 19, 2019
  • Available Online: December 22, 2020
  • Published Date: August 06, 2020
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    • Abstract
  • Objective To explore drug resistance of aneuploid colon cancer cells to cisplatin. Methods The HCT116 cells with expression of knockdown MAD2 induced by doxycycline(DOX)were designed as DOX(+)group(aneuploid), and HCT116 cells without process of DOX were designed as DOX(-)group(euploid). CCK-8 method was used to process cells in both groups, and CCK-8 test and cytometry were used to analyze the effect of cisplatin on cell growth. Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the expression of pro-apoptotic genes BAX, PUMA and NOXA in the cells. Results Cells were treated with cisplatin at different concentrations. CCK-8 test and cytometry showed that cells in DOX(+)group were more resistant to cisplatin than those in DOX(-)group. Western blot showed that the resistance of cells in DOX(+)group was related to the lower expression of apoptotic protein after cisplatin treatment. The qRT-PCR showed that the expressions of apoptosis gene PUMA, NOXA and BAX in DOX(+)group were significantly lower than those in DOX(-)group(P<0.05 or P<0.01)after process of cisplatin. Conclusion The aneuploid colon cancer cells are resistant to cisplatin treatment.
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