Up-regulated expression of synaptophysin by simvastatin through activation of MEK/ERK signaling pathways

YU Chenglu; LYU Tao; MENG Qinggui; WAN Lishu

doi:10.7619/jcmp.20200377

Journal of Clinical Medicine in Practice > 2021 > 25(3): 8-12. > DOI: 10.7619/jcmp.20200377

YU Chenglu, LYU Tao, MENG Qinggui, WAN Lishu. Up-regulated expression of synaptophysin by simvastatin through activation of MEK/ERK signaling pathways[J]. Journal of Clinical Medicine in Practice, 2021, 25(3): 8-12. DOI: 10.7619/jcmp.20200377

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Up-regulated expression of synaptophysin by simvastatin through activation of MEK/ERK signaling pathways

1.
Department of Neurology, Dandong City First Hospital in Liaoning Province, Dandong, Liaoning, 118000
2.
Department of Ophthalmology, Dandong City First Hospital in Liaoning Province, Dandong, Liaoning, 118000

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Received Date: December 24, 2020
Available Online: March 03, 2021
Published Date: March 03, 2021

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Abstract

Abstract

Objective To investigate the effect of simvastatin (SV) on the expression of synaptophysin (SYP) in cerebral cortex of cultured rats in vitro and its signal transduction mechanism.
Methods Neurons were isolated and cultured from cerebral cortex of neonatal Sprague Dawley rats. After 4 days, the rats were divided into control group, SV group (processed with 2, 4, 8 μmol/L SV for 48 h), PD98059 treatment group, and SV combined with PD98059 treatment group (firstly processed with 10 μmol/L blocker PD98059 for 30 min, and then treated with 10 μmol/L SV for 48 h). The expression of SYP was detected by immunofluorescence assay, and the levels of phosphorylated mitogen-activated protein kinase (p-MEK), phosphorylated extracellular regulated protein kinase 1 (p-ERK1) and phosphorylated extracellular regulated protein kinase 2 (p-ERK2) were detected by Western blot.
Results Immunofluorescence assay showed that 4 μmol/L SV could significantly enhance the immunoreactivity of SYP. Western blot showed that 8 μmol/L SV could significantly increase the levels of SYP, p-MEK, p-ERK1 and p-ERK2. PD98059 can significantly reduce up-regulation of SYP, p-MEK, p-ERK1 and p-ERK2 induced by SV.
Conclusion SV can up-regulate SYP expression by activating MEK/ERK signal transduction pathway.
- simvastatin,
- synaptophysin,
- neurons,
- extracellular regulated protein kinase

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Up-regulated expression of synaptophysin by simvastatin through activation of MEK/ERK signaling pathways

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